Protective effect and mechanism of chikusetsu saponin Ⅳa on the kidney in diabetic nephropathy rats
- VernacularTitle:竹节参皂苷Ⅳa对糖尿病肾病大鼠的肾脏保护作用及机制
- Author:
Yongli WANG
1
;
Hai CHEN
1
;
Xiaofang TIAN
1
;
Xuechun WANG
1
;
Liying YUAN
1
;
Dan LIU
1
;
Zhongfa LI
1
;
Yanfang MENG
2
;
Xiuyong YANG
3
Author Information
1. Dept. of Nephrology，the First People’s Hospital of Zunyi，Guizhou Zunyi 563000，China
2. Dept. of Rheumatology and Immunology，the First People’s Hospital of Zunyi，Guizhou Zunyi 563000，China
3. Dept. of Endocrinology，the First People’s Hospital of Zunyi，Guizhou Zunyi 563000，China
- Publication Type:Journal Article
- Keywords:
chikusetsu saponin Ⅳa;
diabetic nephropathy;
insulin resistance;
oxidative stress;
Notch signaling pathway
- From:
China Pharmacy
2026;37(7):908-913
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study the protective effect and potential mechanism of chikusetsu saponin Ⅳ a （chsⅣ） on renal function in diabetic nephropathy （DN） model rats. METHODS DN rat model was established by high-fat diet combined with streptozotocin injection. Thirty-six model rats were randomly divided into model group （i.g. administration of normal saline， high-fat diet）， chsⅣ low-dose and high-dose groups （i.g. administration of 90， 180 mg/kg chsⅣ， high-fat diet）， with 12 rats in each group. Additionally， 10 normal rats were set as the control group （i.g. administration of normal saline， regular diet）. From the 5th to the 12th week after streptozotocin injection， they were given intragastric administration of relevant drug or normal saline， once a day. After the last medication， the levels of fasting blood glucose， fasting insulin， blood urea nitrogen， serum creatinine and urine protein as well as the levels of reduced glutathione （GSH）， superoxide dismutase （SOD） and malondialdehyde （MDA） in renal tissues were measured. Additionally， the insulin resistance index was calculated. Hematoxylin-eosin， periodic acid-Schiff， and Masson staining techniques were employed to examine the histopathological alterations in the renal tissue. The expressions of Notch signaling pathway-related proteins in renal tissue were detected by immunohistochemical staining and Western blot methods. RESULTS Compared with model group， the histomorphological of renal tissues in the chsⅣ low- and high-dose groups were significantly improved， with significant decreases in renal histological scores， mesangial expansion index， and glomerulosclerosis scores （ P ＜0.05）； the levels of fasting blood glucose， fasting insulin， blood urea nitrogen， serum creatinine， urine protein and homeostasis model assessment for insulin resistance， as well as MDA content， the expression levels of Notch1， Notch intracellular domain， hairy and enhancer of Split 1 and Delta-like protein 1 in renal tissue were all significantly decreased （ P ＜0.05）. The levels of GSH and SOD in renal tissue were significantly elevated （ P ＜0.05）. Moreover， the improvement in these indicators was significantly more pronounced in the chsⅣ high-dose group compared to the chsⅣ low-dose group （ P ＜0.05）. CONCLUSIONS ChsⅣ can ameliorate renal pathological damage and functional impairment in DN rats. Its underlying mechanisms include restoration of glucose homeostasis and insulin sensitivity， attenuation of renal oxidative stress， and suppression of aberrant Notch signaling pathway activation.
