Quality control of Sagina japonica by HPLC fingerprint combined with quantitative analysis of multi-components by single-marker
- VernacularTitle:基于HPLC指纹图谱结合一测多评法的漆姑草药材质量控制研究
- Author:
Junhong LIU
1
;
Xue LI
2
;
Meiqin ZHANG
2
;
Han HU
1
;
Chunmei BAI
2
;
Chunhua LIU
3
;
Yongjun LI
2
Author Information
1. Center for Drug Inspection of Guizhou Medical Products Administration，Guiyang 550081，China
2. School of Pharmacy，Guizhou Medical University，Guiyang 561113，China
3. School of Pharmacy，Guizhou Medical University，Guiyang 561113，China;State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine/Engineering Research Center of the Ministry of Education for the Development and Application of Ethnic and Traditional Chinese Medicine/Guizhou Engineering Technology Research Center for the Development and Application of Ethnic and Traditional Chinese Medicine，Guiyang 561113，China
- Publication Type:Journal Article
- Keywords:
Sagina japonica;
fingerprint;
quantitative analysis of multi-components by single-marker;
quality control;
quality
- From:
China Pharmacy
2026;37(7):883-888
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To establish the high-performance liquid chromatography （HPLC） fingerprint of Sagina japonica ， and to establish a quantitative analysis of multi-components by single-marker （QAMS） method for simultaneous determination of six componen ts in S. japonica ， aiming to provide references for the quality control of this medicinal herb. METHODS HPLC method was used to establish the fingerprints of 12 batches （No. S1-S12） of S . japonica according to Similarity Evaluation System of Chromatographic Fingerprint of Traditional Chinese Medicine . The similarity evaluation and identification of common peaks were conducted， followed by cluster analysis （CA） and principal component analysis （PCA） for 12 batches of samples. Using vicenin-2 as internal reference， the contents of p-hydroxy cinnamic acid， apigenin-6-C-arabinoside-8-C-glucoside， isoorientin， vitexin and 20-hydroxyecdysone were determined by QAMS method. The results were then compared with those obtained by the external standard method. RESULTS The similarities of HPLC fingerprints for 12 batches of S . japonica ranged from 0.828-0.998. A total of 17 common peaks were calibrated， and 6 common peaks were identified. Specifically， peak 5 was identified as vicenin-2， peak 7 as p-hydroxycinnamic acid， peak 10 as apigenin-6-C-arabinoside-8-C-glucoside， peak 11 as isoorientin， peak 13 as vitexin， and peak 15 as 20-hydroxyecdysone. The results of CA showed that S1-S5， S7 and S9-S11 were clustered into one category， S6 was clustered into one category， and S8 and S12 were clustered into one category. The results of PCA revealed that the accumulative contribution rate of the four main components was 89.430%. The content ranges measured by QAMS method for p-hydroxy cinnamic acid， apigenin-6-C-arabinoside-8-C-glucoside， isoorientin， vitexin and 20-hydroxyecdysone were 0.017 4-0.269 4， 0.568 8-4.240 3， 0.503 2-5.040 3， 0.024 0-0.132 0 and 2.551 3-4.881 1 mg/g， respectively. There was no significant difference in the contents of components measured between QAMS method and the external standard method （ P ＞0.05）. CONCLUSIONS The established HPLC fingerprint and QAMS method can be used for quality evaluation and quality control of S . japonica.
