Effects of galangin on rheumatoid arthritis in rats by regulating the JAK3/STAT3 pathway
- VernacularTitle:高良姜素调控JAK3/STAT3通路对大鼠类风湿性关节炎的影响
- Author:
Yan HUANG
1
;
Weiming WANG
2
;
Haiying LIU
3
;
Yi ZHAN
1
;
Xi CHEN
4
;
Dehong YU
1
Author Information
1. Dept. of Rheumatology and Immunology，the Affiliated Hongqi Hospital of Mudanjiang Medical University，Heilongjiang Mudanjiang 157000，China
2. Dept. of Respiratory and Critical Care Medicine，the Affiliated Hongqi Hospital of Mudanjiang Medical University，Heilongjiang Mudanjiang 157000，China
3. Dept. of Integrated Traditional Chinese and Western Medicine，the Affiliated Hongqi Hospital of Mudanjiang Medical University，Heilongjiang Mudanjiang 157000，China
4. Dept. of Gastroenterology，the Affiliated Hongqi Hospital of Mudanjiang Medical University，Heilongjiang Mudanjiang 157000，China
- Publication Type:Journal Article
- Keywords:
galangin;
rheumatoid arthritis;
autophagy;
apoptosis;
synovial cell proliferation;
inflammatory response;
JAK3/
- From:
China Pharmacy
2026;37(6):764-769
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the effects of galangin on rheumatoid arthritis （RA） in rats by regulating the Janus kinase 3 （JAK3）/signal transducer and activator of transcription 3 （STAT3） pathway. METHODS Fifty male SD rats were taken， and an emulsion composed of bovine type Ⅱ collagen and Freund’s complete adjuvant was injected subcutaneously to establish an induced arthritis model. The rats that were successfully modeled were randomly divided into model group， low， medium and high dose groups of galangin （1， 5， 15 mg/kg）， and methotrexate group （positive control， 2 mg/kg）， with 10 rats in each group. Another 10 normal rats were taken as the normal group. Starting from the 15th day of modeling， each group of rats was gavaged with the corresponding drug solution or normal saline containing 0.5% Tween 80 once a day for 28 consecutive days. The arthritis index （AI） scores and paw volume of rats were compared before and after gavage administration. Twenty-four hours after the last administration， the serum levels of interleukin-6 （IL-6）， tumor necrosis factor-α （TNF-α）， IL-4 and IL-10 were determined， the pathological changes in ankle joint synovial tissue were observed， and the protein expressions of UNC-51 like kinase 1 （ULK1）， Beclin-1， microtubule-associated protein 1 light chain 3 （LC3）， B cell lymphoma 2 （Bcl-2）， Bcl-2-associated X protein （Bax）， caspase-3， JAK3， phosphorylated JAK3 （p-JAK3）， STAT3 and phosphorylated STAT3 （p-STAT3） in the synovial tissue of the ankle joint were detected， as well as the fluorescence intensity of LC3-positive areas. RESULTS Compared with the model group， the pathological changes such as cellular proliferation of ankle joint synovial tissue and infiltration of inflammatory cells in rats of each administration group showed improvement. Moreover， their AI scores and paw pad volumes （on day 28 after gavage）， the levels of IL-6 and TNF-α， the protein expression of Bcl-2， and the phosphorylation levels of JAK3 and STAT3 were all significantly reduced （ P ＜0.05）. The levels of IL-4 and IL-10， the protein expressions of ULK1， Beclin-1， Bax， caspase-3 and LC3， as well as the fluorescence intensity of LC3-positive areas， were all significantly increased （ P ＜0.05）. Moreover， the effect of galangin was in a dose-dependent manner （ P ＜0.05）. CONCLUSIONS Galangin can induce sustained autophagy in synovial tissue cells of RA rats， promote cell apoptosis， inhibit synovial cell proliferation， and alleviate persistent inflammatory responses. The above anti-RA effects may be related to the inhibition of the JAK3/STAT3 pathway.
