Intervention effect of nicotinamide mononucleotide on subacute benzene exposure-induced hematotoxicity in mice
10.20001/j.issn.2095-2619.20251202
- VernacularTitle:烟酰胺单核苷酸对亚急性苯染毒致小鼠血液毒性干预作用
- Author:
Haohan CHEN
1
;
Hongyun CHEN
;
Xiaoyu MAO
;
Zongxin LI
;
Xiaolin LUO
;
Mengjun HOU
;
Qin XIAO
;
Yongmei XIAO
;
Xiumei XING
Author Information
1. School of Public Health, Sun Yat-sen University, Guangzhou, Guangdong 510080, China
- Publication Type:Journal Article
- Keywords:
Benzene;
Hematotoxicity;
Nicotinamide mononucleotide;
Leukocytes;
Neutrophil;
Platelet;
Intervention effect;
Mouse
- From:
China Occupational Medicine
2025;52(6):612-617
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assess the intervention effect of nicotinamide mononucleotide (NMN) on the mouse model of hematotoxicity induced by subacute benzene exposure. Methods Benzene exposure and NMN intervention were adopted in a 2×2 factorial design, as benzene exposure and non-exposure, and NMN intervention and non-intervention. Male specific pathogen-free C57BL/6J mice were randomly assigned to negative control group, NMN control group, simple benzene exposure group and NMN intervention group, with 12 mice in each group. Benzene exposure of mice in simple benzene exposure group and NMN intervention group was conducted by dynamic inhalation of benzene at a concentration of 325 mg/m³ for six hours per day, five days per week for four weeks (28 days). Mice in the negative control and NMN control group inhaled clean air. During benzene exposure, mice in the NMN control group and NMN intervention group received NMN in drinking water at a dose of 300 mg/kg body weight. Peripheral blood samples of mice were collected for complete blood count analysis and calculation of composite inflammatory indices after 28 days. Results Interaction analysis showed that the counts of peripheral white blood cell, neutrophil, lymphocyte, and platelet of mice in the simple benzene exposure group were lower than those in the negative control group (all P<0.05). Neutrophil and platelet counts in the NMN intervention group were higher than those in the simple benzene exposure group (all P<0.05). The results of main effect analysis showed that the monocyte count of peripheral blood, systemic inflammatory index, systemic inflammatory response index, neutrophil/lymphocyte ratio and platelet/lymphocyte ratio of mice in the benzene exposure group increased (all P<0.05), and the basophil count and lymphocyte/monocyte ratio decreased (all P<0.05), compared with the control group. Conclusion Oral NMN alleviates subacute benzene-induced decreases in peripheral neutrophil and platelet counts in mice. This protective effect may be related to the targeted intervention of NMN on mitochondrial energy metabolism disorder and oxidative damage induced by benzene exposure in male mice.
