Transcriptome-based Mining of Genes Involved in Regulation of Cyclopeptide B Synthesis in Pseudostellaria heterophylla
10.13422/j.cnki.syfjx.20260214
- VernacularTitle:基于转录组挖掘太子参环肽B合成调控相关基因
- Author:
Qingsu ZHOU
1
;
Yishu HUANG
1
;
Xiuwen WANG
1
;
Jiao XU
1
;
Xiaohong OU
1
;
Hua HE
1
;
Weike JIANG
1
;
Tao ZHOU
1
Author Information
1. Guizhou University of Traditional Chinese Medicine， Guiyang 550025，China
- Publication Type:Journal Article
- Keywords:
Pseudostellaria heterophylla;
heterophyllin B;
transcriptome;
transcription factors;
gene regulation
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2026;32(9):224-230
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveThe biosynthesis of heterophyllin B （HB）， a cyclopeptide from Pseudostellaria heterophylla， is regulated by various abiotic stresses. Elucidating the transcriptional regulatory mechanism underlying HB biosynthesis is of great guiding significance for the directional improvement of P. heterophylla varieties and the enhancement of HB content. MethodsBased on transcriptome data from different tissues of P. heterophylla， transcription factors （TFs） specifically upregulated and highly expressed in the phloem of tuberous roots were screened through a combination of Mfuzz time-series clustering， transcription factor family prediction， and correlation analysis. Quantitative real-time polymerase chain reaction （Real-time PCR） was employed to analyze expression patterns of candidate TFs under abscisic acid （ABA） induction， and the dual-luciferase reporter assay was applied to verify their regulatory effects on HB precursor genes. ResultsContent determination showed that HB accumulated at the highest in the phloem of P. heterophylla tuberous roots （34 μg·g-1 fresh weight）. Transcriptome analysis identified 15 868 up-regulated differentially expressed genes （DEGs）， among which 4 375 were specifically up-regulated in the phloem. From these， 25 TFs highly expressed in the phloem were screened. Correlation analysis revealed that the expression level of WRKY70 was significantly positively correlated with HB content， the expression of precursor genes prePhHB， and PhPOP1. Additionally， WRKY70 expression was significantly upregulated under ABA induction. Dual-luciferase reporter assay confirmed that WRKY70 specifically activated the transcriptional activity of the prePhHB promoter （Pro-prePhHB）. ConclusionHB is mainly accumulated in the phloem of P. heterophylla tuberous roots. WRKY70 positively regulates HB biosynthesis by directly activating the promoter activity of prePhHB. This study clarifies the key role of WRKY70 in the regulation of HB biosynthesis and provides an important theoretical basis for the in-depth analysis of the molecular regulatory mechanism underlying HB biosynthesis.
