Study on Reducing Hepatotoxicity and Retaining Anti-osteoporosis Activity of Psoraleae Fructus Though Salt Processing Based on Zebrafish
10.13422/j.cnki.syfjx.20260213
- VernacularTitle:基于斑马鱼幼鱼的补骨脂盐炙减毒存效探索
- Author:
Yiqi LIU
1
;
Xuan WANG
1
;
Qiqi FAN
1
;
Zehua CHANG
1
;
Shuo FAN
1
;
Na WANG
1
;
Zheng LI
1
;
Xinfang XU
1
;
Chongjun ZHAO
1
;
Xiangri LI
1
Author Information
1. School of Chinese Materia Medica Beijing University of Chinese Medicine/Beijing Key Laboratory for Quality Evaluation of Chinese Materia Medica， Beijing 100102， China
- Publication Type:Journal Article
- Keywords:
Psoraleae Fructus;
salt processing;
zebrafish;
hepatotoxicity;
anti-osteoporosis activity
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2026;32(9):79-90
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the mechanism of salt processing of Psoraleae Fructus （PF） through modern analytical techniques and biotechnology， focusing on its effects related to hepatotoxicity and anti-osteoporosis activity. MethodsThe zebrafish model was utilized to evaluate the impact of PF and salt-processed Psoraleae Fructus （SPF） on the hepatotoxicity （using 134.17 ， 178.89， 268.34 mg·L-1 as low， medium， and high dose groups of PF， 135.04， 180.06， 270.08 mg·L-1 as low， medium， and high dose groups of SPF， respectively） and anti-osteoporotic activity （using 33.54 ， 67.08 and 134.17 mg·L-1 as low， medium， and high dose groups of PF， 33.76， 67.52， 135.04 mg·L-1 as low， medium， and high dose groups of SPF， respectively）， which was using alizarin red skull staining of zebrafish as an indicator of different batches of PF. The specific dosage of a batch of PF was taken as an example. Then ultra-performance liquid chromatography-quadrupole-time of flight-mass spectrometry（UPLC-Q-TOF-MS） analysis was employed to identify the chemical composition of PF before and after salt processing， and PCA， OPLS-DA， and independent sample t-test were used to elucidating the compositional changes associated with the effects of salt processing on hepatotoxicity and anti-osteoporosis activity. ResultsUnder specific conditions， PF induced notable hepatotoxicity in zebrafish while simultaneously demonstrating protective effect against prednisolone-induced osteoporosis. In comparison to PF， SPF showed alleviated hepatotoxicity while retaining significant anti-osteoporosis activity. UPLC-Q-TOF-MS analysis revealed that after salt processing， the overall chemical composition of PF showed a downward trend， with 69 components showing a decrease in content， represented by psoralen， and 13 components showing an increase， represented by 4′-O-methyl psoralen B. Further multivariate statistical analysis revealed 11 key differential components before and after salt processing of PF， including psoralen and bakuchiol. ConclusionSalt processing effectively diminishes hepatotoxicity without impairing therapeutic efficacy against osteoporosis of PF， which may be related to the compositional changes before and after salt processing of PF and provides key evidence to reveal the scientific significance of salt processing of PF.
