Hemolytic phenotype analysis of Staphylococcus aureus clinical isolates
10.19405/j.cnki.issn1000-1492.2025.07.021
- VernacularTitle:金黄色葡萄球菌临床分离株的溶血表型研究
- Author:
Ju Gao
1
;
Shengnan Weng
1
;
Guiyun Leng
1
;
Xin Li
1
;
Jie Yao
1
;
Qiang Zhou
1
;
Wei Tang
1
Author Information
1. Dept of Clinical Laboratory，The Second Affiliated Hospital of Anhui Medical University，Hefei 230601
- Publication Type:Journal Article
- Keywords:
Staphylococcus aureus;
clinical isolate;
hemolytic phenotype;
hemolysin;
antibiotic resistance;
virulence
- From:
Acta Universitatis Medicinalis Anhui
2025;60(7):1312-1319
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the hemolytic phenotypes of Staphylococcus aureus clinical isolates．
Methods:The hemolytic phenotypes of 105 Staphylococcus aureus isolates were analyzed and summarized using the three-point inoculation method．Real-time fluorescence quantitative PCR was used to measure the mRNA expression levels of four hemolysin genes (hla，hlb，hlc，and hld) ; The VITEK 2 GP639 antimicrobial susceptibility card was used to detect resistance to commonly used antibiotics ; DNA gel electrophoresis was performed to determine the prevalence of the mecA，sea，tst，and pvl genes ; The microtiter plate crystal violet staining method was used to assess biofilm formation ability ; The CCK-8 assay was used to evaluate cytotoxicity against macrophages．
Results:Seven hemo- lytic phenotypes were identified among the Staphylococcus aureus clinical isolates． Differences were found among Staphylococcus aureus clinical isolates with different hemolytic phenotypes in terms of mRNA expression levels of he- molysin genes，antibiotic resistance，virulence gene prevalence，biofilm formation ability，and cytotoxicity to mouse macrophages (P ＜0. 05 ) ．
Conclusion:Staphylococcus aureus clinical isolates exhibit diverse hemolytic pheno- types，which should be a focus across multiple dimensions，including microbiological testing，clinical treatment， and nosocomial infection prevention and control．
- Full text:2026041423090982039金黄色葡萄球菌临床分离株的溶血表型研究_高菊.pdf
