The effects of IgD on the proliferation and apoptosis of acute myeloid leukemia cells Molm-13
- VernacularTitle:IgD 对急性髓系白血病细胞 Molm⁃13 增殖与凋亡的影响
- Author:
Danyan Liu
1
;
Xin Zhang
1
;
Mengqin Chen
1
;
Xi Ling
1
;
Manling Dong
1
;
Tiantian Wu
1
;
Yueye Wang
1
;
Tao Li
2
;
Wei Wei
1
;
Yujing Wu
1
Author Information
- Publication Type:Journal Article
- Keywords: acute myeloid leukemia; IgD; Molm ⁃13; proliferation; apoptosis
- From: Acta Universitatis Medicinalis Anhui 2025;60(8):1513-1519,1534
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the role and related mechanisms of IgD on the viability , proliferation , apoptosis , and other functions of Molm_13 cells.
Methods:Peripheral blood serum was collected from AML patients and healthy controls. The sIgD levels were quantified by ELISA. For in vitro studies , Molm_13 cells were treated with varying concentrations of IgD. Cell viability and proliferation were assessed via CCK_8 assays , CFSE staining , and colony formation assays. Apoptosis rates were determined using an Annexin V/PI apoptosis detection kit. Preliminary exploration of the mechanisms related to IgD_induced proliferation of Molm_13 were analyzed through differential gene analysis.
Results:Compared with healthy controls , the levels of sIgD in AML patients were significantly el_ evated (P < 0. 001 ) . IgD treatment dose_dependently increased Molm_13 cell viability and proliferation ( P < 0. 05) , inhibited apoptosis rates (P < 0. 001) .
Conclusion :IgD promotes the viability and proliferation of Molm_ 13 cells , and reduces apoptosis. - Full text:2026041115051779587IgD对急性髓系白血病细胞Molm-13增殖与凋亡的影响_刘丹彦.pdf
