Zerumbone attenuates cisplatin-induced acute kidney inj ury in mice

Xiufeng Luo; Manman Xie; Runrun Shan; Chunya Xie; Jiaozhuang Liu; Liangting Liu; Shaofei Zhang; Qi Chen

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Zerumbone attenuates cisplatin-induced acute kidney inj ury in mice

VernacularTitle:花姜酮减轻顺铂诱导的小鼠急性肾损伤
Author: Xiufeng Luo ¹ ; Manman Xie ¹ ; Runrun Shan ¹ ; Chunya Xie ¹ ; Jiaozhuang Liu ¹ ; Liangting Liu ¹ ; Shaofei Zhang ² ; Qi Chen ¹
Author Information

1. School of Life Sciences , Anhui Medical University, Hefei 230032
2. School of Pharmacy,Anhui Medical University, Hefei 230032
Publication Type:Journal Article
Keywords: zerumbone; cisplatin; acute kidney injury; inflammation; necroptosis
From: Acta Universitatis Medicinalis Anhui 2025;60(8):1454-1462
CountryChina
Language:Chinese
Abstract: Objective:To investigate whether zerumbone ( ZER) has the effect of preventing cisplatin ( Cis) -induced acute kidney injury (Cis-AKI) .
Methods:The MTT method was used to detect the effect of different concentrations of ZER on the cell viability of Cis-AKI. The in vivo and in vitro models of Cis-AKI mice were estab- lished by dividing into control group , separate administration group , model group , and dose group. Western blot and immunofluorescence experiments were used to detect the expression changes of kidney injury marker-1 ( KIM- 1) , phosphorylated NF-κB p65 ( P-p65 ) , Cleaved casepase3 , receptor interacting protein kinase 1 ( RIPK1) , RIPK3 , and tumor necrosis factor-α (TNF-α) . Real-time fluorescence quantitative PCR (qRT-PCR) was used to detect the mRNA expression of KIM-1 , TNF-α , interleukin-6 ( IL-6) , and monocyte chemoattractant protein-1(MCP-1) . Periodic acid-Schiff (PAS) staining confirmed the therapeutic effect of ZER on Cis-AKI. RNA-seq and cell thermal shift (CETSA) were used to explore possible target proteins.
Results :MTT results showed that ZER could alleviate the decrease in cell viability of Cis-AKI ; in vivo and in vitro studies showed that compared with the model group , after treatment with ZER , its KIM-1 , P-p65 , Cleaved casepased3 , RIPK1 , RIPK3 , TNF -α expres- sion decreased significantly , and the mRNA expression of KIM-1 , TNF-α , IL-6 mRNA , and MCP-1 decreased. PAS staining showed that ZER had a therapeutic effect on Cis-AKI. RNA-seq and CETSA analysis showed that ZER might prevent and treat Cis-AKI by targeting the PIM1 protein.
Conclusion :ZER may alleviate Cis-AKI and im- prove inflammatory response and necroptosis by regulating PIM1 protein. ZER is expected to be a potential drug for the prevention and treatment of Cis-AKI.
Full text:2026040500231978190花姜酮减轻顺铂诱导的小鼠急性肾损伤_罗秀峰.pdf