Comparative transcriptome profiling of three different murine modelsof metabolic dysfunction-associated steatohepatitis
10.19405/j.cnki.issn1000-1492.2025.08.012
- VernacularTitle:三种不同代谢紊乱相关脂肪性肝炎小鼠模型 的转录组比较分析
- Author:
Tianwen Liu
1
;
Ziyi Guo
2
;
Hanqi Bi
3
;
Bing Zhou
3
;
Yan Lu
3
;
Fei Mao
4
;
Hua Wang
1
Author Information
1. Dept of Oncology , The First Afiliated Hospital of Anhui Medical University , Hefei 230022; Inflammation and Immune⁃mediated Diseases Laboratory of Anhui Province , Anhui Medical University , Hefei 230032
2. Dept of Endocrinology and Metabolism , Minhang Hospital , Fudan University , Shanghai 201199
3. Institute of Metabolism and Regenerative Medicine , Digestive Endoscopic Center , Shanghai Sixth People ′s Hospital Afiliated to Shanghai Jiao Tong University School of Medicine , Shanghai 200233
4. Dept of Endocrinology and Metabolism , Zhongshan Hospital , Fudan University , Shanghai 200032
- Publication Type:Journal Article
- Keywords:
MASH;
murine models;
transcriptome;
comparative analysis;
differentially expressed genes;
meta⁃ bolic pathway
- From:
Acta Universitatis Medicinalis Anhui
2025;60(8):1445-1453
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To compare the transcriptomic profiles between three distinct metabolic dysfunction⁃associat⁃mal murine model that more closely resembles human MASH progression .
Methods:Forty 8 ⁃week⁃old male C57BL/6J mice were randomly assigned to either a control group fed normal chow diet ( NCD) or one of three MASH model groups receiving high⁃fat high⁃cholesterol diet (HFHCD) , choline⁃deficient high⁃fat diet (CDHFD) ,from three randomly selected mice per group were collected for mRNA sequencing ( mRNA⁃seq) analysis . Mean⁃bases . Overlap of functional profiles was analyzed by gene set enrichment analysis (GSEA) profiles to compare the mouse transcriptome with that of human patients at different stages of the disease . Additionally , Pearson ′s correla⁃tion analysis was used to explore the correlation between gene expression of murine models and human MASH .
Results:Seven commonly up⁃regulated genes (Col1a1 , Smoc2 , Col6a1 , Gpx3 , Col16a1 , Spp1 and Crtap) were de⁃ways involving steatosis , hepatocellular injury and fibrosis were detected in the three MASH models at the pathway level . HFHCD and MCD might share more common traits . In comparing gene expression and pathway profiles be⁃tween different murine models and patients with different stages of MASH , all three murine MASH models showed a closer resemblance to the human progressive stages of MASH . Notably , the transcriptomic features of the CDHFD model were more consistent with those of human MASH .
Conclusion:There are certain similarities and differences among the transcriptional profiles of the three MASH models . The MASH models are more similar to the advanced stage of MASH in human patients . Compared to the other two models , the CDHFD model ′ s transcriptome profile more closely resembles human MASH .
- Full text:2026040500181411284三种不同代谢紊乱相关脂肪性肝炎小鼠模型的转录组比较分析_刘天雯.pdf
