Protective effect of dulaglutide on lipopolysaccharide- induced MLE-12 cells
10.19405/j.cnki.issn1000-1492.2025.08.011
- VernacularTitle:度拉糖肽对脂多糖诱导的 MLE⁃12 细胞损伤的保护作用
- Author:
Xingyu Fan
1
;
Hao Duan
1
;
Jie Yan
1
;
Yue Wang
1
;
Yijun Du
1
;
Tianrong Pan
1
;
Xing Zhong
1
Author Information
1. Dept of Endocrinology, The Second Afiliated Hospital of Anhui Medical University, Hefei 230601
- Publication Type:Journal Article
- Keywords:
dulaglutide;
lipopolysaccharide;
acute lung injury;
MLE-12 cells;
inflammatory response
- From:
Acta Universitatis Medicinalis Anhui
2025;60(8):1439-1444
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the protective effect of dulaglutide on lipopolysaccharide (LPS)-induced inju- ry in MLE-12 cells.
Methods:An in vitro model of acute lung injury was established by inducing MLE-12 cells with LPS ( 1 μg/mL) , followed by treatment with dulaglutide for 24 hours. The cells were divided into four groups : CON group , LPS group , LPS + 100 nmol/L dulaglutide group , and LPS + 200 nmol/L dulaglutide group. Protein and RNA were extracted from each group. The mRNA levels of inflammatory factors , including interleukin (IL)-6 , tumor necrosis factor-α (TNF-α ) , IL-1β , monocyte chemotactic protein 1 (CCL2) , C-X-C motif chemokine lig- and (CXCL) 1 and CXCL2 , were detected by qRT-PCR. Cell apoptosis was assessed by TUNEL assay , and the expression levels of phosphorylated protein kinase B (P-Akt) and phosphorylated extracellular signal-regulated ki- nase (P-Erk) were measured by Western blot.
Results:Compared with the CON group , the LPS group showed in- creased mRNA levels of inflammatory mediators (TNF-α , IL-6 , IL-1β , CCL2 , CXCL1 , and CXCL2) , increased TUNEL-positive cells , and elevated expression of P-Akt and P-Erk proteins. Compared with the LPS group , the LPS + 100 nmol/L dulaglutide treatment group exhibited reduced mRNA levels of TNF-α , IL-6 , IL-1β , CCL2 , CXCL1 , and CXCL2 , decreased TUNEL-positive cells , and downregulated expression of P-Akt and P-Erk pro- teins. However, the LPS + 200 nmol/L dulaglutide treatment group showed less pronounced improvement in inflam- matory factors compared to the LPS + 100 nmol/L dulaglutide group.
Conclusion:Dulaglutide has a protective effect on LPS-induced injury in MLE-12 cells , potentially through inhibiting Akt and Erk phosphorylation , thereby reducing the expression of inflammatory mediators and alleviating inflammatory damage , ultimately protecting the lungs.
- Full text:2026040500133392333度拉糖肽对脂多糖诱导的MLE-12细胞损伤的保护作用_范星宇.pdf
