Fecal microbiota transplantation regulates the composition of intestinal-derived estrogens and their metabolites : a study on the correlation with non-alcoholic fatty liver disease
10.19405/j.cnki.issn1000-1492.2025.08.009
- VernacularTitle:粪菌移植调节肠源性雌激素及其代谢物组成 与非酒精性脂肪性肝病相关性研究
- Author:
Wanli Li
1
;
Xueping Qi
2
;
Shuqi Cong
1
;
Wanting Zhang
1
;
Tingting Zhang
1
;
Sheng Wang
3
;
Haiming Fang
4
;
Jiyue Wen
1
;
Jiajia Wang
1
Author Information
1. Dept of Pharmacology, School of Basic Medical Sciences , Anhui Medical University, Hefei 230032
2. Dept of Pharmacy, Mingji Hospital , Nanjing 210019
3. Center for Scientific Research , Anhui Medical University, Hefei 230032
4. Dept of Gastroenterology, The Second Afiliated Hospital of Anhui Medical University, Hefei 230601
- Publication Type:Journal Article
- Keywords:
non-alcoholic fatty liver disease;
gut microbiota;
fecal microbiota transplantation;
estrogens;
metabo- lomics
- From:
Acta Universitatis Medicinalis Anhui
2025;60(8):1423-1431
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the impact of fecal microbiota transplantation (FMT) on the composition of 15 intestinal-derived estrogens and their metabolites (EMs) and its correlation with non-alcoholic fatty liver disease (NAFLD) .
Methods:Thirty male C57BL/6J mice were divided into a normal control group (Control) , a high- sugar high-fat diet combined with low-dose CCl4 -induced NAFLD model group ( Model) , and a group of model mice treated with fecal microbiota from normal female mice (FMT) . After 17 weeks of modeling , liver pathology in each group was observed using HE staining , biochemical methods were used to measure serum alanine aminotrans- ferase (ALT) and aspartate aminotransferase (AST) levels , as well as hepatic triglyceride (TG) and total choles- terol (TC) levels. and the content of 15 EMs in portal vein serum was detected using ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS) . The correlation between disease phenotype and intesti- nal EMs was analyzed using Pearson ′s method.
Results:The NAFLD model was successfully established , and the FMT group showed improved liver structure and morphology , with significant decreases in liver function and hepatic lipids compared to the Model group. In NAFLD mice , the contents of E1 , E2 , and their 2- and 4-position metabo- lites in portal vein blood serum was reduced compared to normal mice , while the content of most 16- and 17-posi- tion metabolites ( except 16α-OHE1) increased compared to normal mice. Correlation analysis showed that ALT was strongly positively correlated with E3 and 17-epiE3 , and strongly negatively correlated with E1 , E2 , 4- MeOE1 , and 16α-OHE1 . The TC was strongly positively correlated with 17-epiE3 and strongly negatively correla- ted with E1 , 4-MeOE1 , and 16α-OHE1 .
Conclusion:FMT restores the disrupted composition of intestinal EMs and improves NAFLD.
- Full text:2026040500061679989粪菌移植调节肠源性雌激素及其代谢物组成与非酒精性脂肪性肝病相关性研究_李婉丽.pdf
