Changes in behavior and spatial memory of C57BL/6J mice of different ages
10.19405/j.cnki.issn1000-1492.2025.08.007
- VernacularTitle:不同年龄段 C57BL/6J 雌性小鼠的行为及空间记忆变化
- Author:
Zhen Yang
1
;
Hongmei Bai
1
;
Weikang Hu
1
;
Mingcong Li
2
;
Xiaoli Jiang
1
;
Chaoyang Zhang
3
;
Zihan Wang
1
;
Wenjing Zhou
1
;
Qingya He
1
;
Jian Zhong
1
;
Shengquan Zhang
1
Author Information
1. Dept of Biochemistry and Molecular Biology , School of Basic Medicine
2. Dept of Pathology , Anhui Medical University Afiliated Hefei Hospital ( The Second People ′s Hospital of Hefei) , Hefei 230022
3. Center for Scientific Research , Anhui Medical University , Hefei 230032
- Publication Type:Journal Article
- Keywords:
C57BL/6J mice;
female rats;
spatial memory;
anxiety;
CaMKⅡ(p);
ethology
- From:
Acta Universitatis Medicinalis Anhui
2025;60(8):1410-1417
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To explore the changes in behavior and spatial memory of C57BL/6J female mice of different ages (youth , middle-aged , and elderly) .
Methods:C57BL/6J female mice were divided into female youth group (YG group) , female middle-aged group ( MG group) and female elderly group ( OG group) according to age. The Morris water maze test measured spatial memory ability , and the open field and elevated cross maze test observed activity level and anxiety level. Western blot was used to determine the protein expressions of CREB , CaMKⅡ(pan) and CaMKⅡ(p) in the hippocampus of the brain tissues of female mice in each group.
Results:Compared with the YG group , the weight of the MG group and the OG group significantly increased (P < 0. 01 , P < 0. 001) . Compared with the OG group , the third quadrant escape latency and the number of crossings in the YG group and MG group were shortened , and the difference was not statistically significant. Compared with the OG group , there was a statistically significant difference in the exercise speed in the open field of the YG group (P < 0. 01) , there was no significant difference in the movement speed in the open field of the MG group , the number of entries into the central zone significantly increased in the MG group ( P < 0. 05 ) , and there was no significant difference in the number of entries in the YG group (P > 0. 05) . Compared with the OG group , the YG group had a statistically significant difference in the elevated cross maze (P < 0. 05) , the MG group had no statistically signif- icant difference in the elevated cross maze , and the number of closed arm entries in the YG group and MG group significantly increased (P < 0. 001 , P < 0. 01) . Compared with the YG group , the relative expression level of CaMKⅡ(pan) in the OG group was statistically significant ( P < 0. 05 ) , while the relative expression level of CaMKⅡ(pan) in the MG group was not statistically significant ( P > 0. 05) .
Conclusion:With the increase of age , the weight of C57BL/6J female mice gradually increased , the activity level and desire to explore gradually de- creased , the spatial memory ability also declined , and the anxiety level and anxiety-like behavior increased. This study helps to reveal the effect of age on the activity level and cognitive function of females , and provides a refer- ence for studying cognitive and memory decline in older females.
- Full text:2026040423483426119不同年龄段C57BL_6J雌性小鼠的行为及空间记忆变化_杨振.pdf
