The cell biological function , clinical significance and prognosis values of DHX32 in pancreatic cancer
10.19405/j.cnki.issn1000-1492.2025.08.005
- VernacularTitle:DHX32 在胰腺癌中的功能 、临床意义及预后价值
- Author:
Dandan Ma
1
;
Qinquan Zhang
1
;
Yi Dong
1
;
Zhonghu Li
1
;
Zhiyong Zhang
1
Author Information
1. Dept of General Surgery , General Hospital of Central Theater Command , Wuhan 430070
- Publication Type:Journal Article
- Keywords:
DHX32;
pancreatic carcinoma;
function;
clinical significance;
prognosis
- From:
Acta Universitatis Medicinalis Anhui
2025;60(8):1393-1400
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the expression and prognosis of DHX32 in pancreatic cancer and observe the biological function of DHX32 in pancreatic cancer cell line SW1990 . To investigate the clinical significance and prognostic value of DHX32 in pancreatic cancer , and predict the possible related mechanism.
Methods :GEPIA database was used to analyze the expression and prognosis of DHX32 in pancreatic cancer , and TCGA database was used to analyze the relationship between DHX32 mRNA expression level and clinicopathological features and prognosis of patients with pancreatic cancer. The effects of DHX32 on the proliferation , invasion and migration of SW1990 pancreatic cancer cells were studied by EdU immunofluorescence assay , Transwell assay and scratch assay. GSEA was used to predict DHX32 the possible related signaling pathways involved with DHX32 in pancreatic cancer.
Results:The results of GEPIA database analysis showed that the expression level of DHX32 in pancreatic cancer tumor tissues was significantly higher than that in normal pancreatic tissues ( P < 0. 05 ) . Meanwhile , the prognosis of pancreatic cancer patients with high DHX32 expression was worse than that of patients with low DHX32 expression (P < 0. 001) . Cox regression analysis showed that DHX32 mRNA expression level and M stage were independent risk factors for prognosis of pancreatic cancer patients (all P < 0. 05) . EdU immunofluorescence assay , Transwell assay and scratch assay confirmed that the overexpression of DHX32 promoted the proliferation , invasion and migration of pancreatic cancer cells (all P < 0. 001) , while silencing DHX32 inhibited the proliferation , invasion and migration of pancreatic cancer cells ( all P < 0. 001) . The results of GSEA enrichment analysis showed that DHX32 was enriched in four signaling pathways : hematopoietic cell lineage , neuroactive ligand receptor interaction , primary immune deficiency and spliceosome (all P < 0. 05) .
Conclusion:DHX32 is highly expressed in pancreatic cancer. It promotes the proliferation , invasion and metastasis of pancreatic cancer cells , and is closely related to the poor prognosis of pancreatic cancer patients .
- Full text:2026040323080868842DHX32在胰腺癌中的功能、临床意义及预后价值_马丹丹.pdf
