Effect of intra-articular hemorrhage on extending knee joint contracture in rats
10.19405/j.cnki.issn1000-1492.2025.08.003
- VernacularTitle:关节内出血对大鼠伸直型膝关节挛缩的影响
- Author:
Quanbing Zhang
1
;
Deting Zhu
1
;
Yun Zhou
1
;
Xiuli Kan
1
;
Lei Huo
2
;
Ruoxi Zhang
3
;
Han Xiao
1
;
Jing Mao
1
;
Xueming Li
4
;
Run Zhang
1
Author Information
1. Dept of Rehabilitation Medicine , The Second Afiliated Hospital of Anhui Medical University, Hefei 230601;The Second Clinical School of Medicine , Anhui Medical University, Hefei 230601
2. Dept of Rehabilitation Medicine , The Second Afiliated Hospital of Anhui Medical University, Hefei 230601
3. The Second Clinical School of Medicine , Anhui Medical University, Hefei 230601
4. Dept of Rehabilitation Medicine , The Second Afiliated Hospital of Anhui Medical University, Hefei 230601;The Second Clinical School of Medicine , Anhui Medical University, Hefei 230601Dept of Rehabilitation Medicine , The Second Afiliated Hospital of Anhui Medical University, Hefei 230601;The Second Clinical School of Medicine , Anhui Medical University, Hefei 230601
- Publication Type:Journal Article
- Keywords:
knee joint contracture;
fibrosis;
knee joint range of motion;
joint hemorrhage;
Wnt/β⁃catenin signa⁃ ling pathway
- From:
Acta Universitatis Medicinalis Anhui
2025;60(8):1381-1386
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of intraarticular hemorrhage on extending knee joint contracture model in rats .
Methods:18 mature male SD rats were divided into 3 groups by random number table method . The control group ( group C) was not immobilized and was killed after 4 weeks of feeding . In the simple fixation group( M1 group) , the left lower limb knee joint was immobilized in straight position for 4 weeks . The blood fixationgroup (M2 group) was injected into the knee cavity with body blood and immobilized in a straight position for 4 weeks . The knee joint motion of each group was measured by the joint motion measuring instrument under a stand⁃ard torque . The contracture degree was calculated by the joint range of motion of the knee joint before and after muscles separation . HE staining and Masson staining were used to detect the number of cells and collagen deposi⁃tion in the anterior joint capsule . The protein expressions of transforming growth factor 1 (TGF⁃ β1) , wingless⁃type MMTV integration site family , member 1 ( Wnt1) and beta⁃catenin ( β⁃catenin) in the anterior articular capsule were detected by Western blotting .
Results:Compared with group C , total knee contracture and arthrogenic con⁃tracture of rats in M1 and M2 groups increased , and the difference was statistically significant (P < 0. 05) . At the same time , the degree of total contracture and arthrogenic contracture in M2 group was higher than that in M1 group , and the difference was statistically significant (P < 0. 05) . Compared with group C , the number of anterior joint capsule cells and collagen deposition in M1 and M2 groups increased , and the difference was statistically sig⁃group were higher than those in M1 group , and the difference was statistically significant (P < 0. 05) . Compared with group C , the protein expressions of TGF⁃ β1 , Wnt1 and β ⁃catenin in the anterior articular capsule of rats in M1 expressions of TGF⁃ β1 , Wnt1 and β ⁃catenin in the anterior articular capsule of the knee joint in M2 group were sig⁃nificantly higher than those in M1 group , with statistical significance (P < 0. 05) .
Conclusion:Joint immobiliza⁃ tion can lead to joint contracture , and joint bleeding aggravates the degree of joint capsule fibrosis induced by im⁃mobilization .
- Full text:2026040322062083390关节内出血对大鼠伸直型膝关节挛缩的影响_张全兵.pdf
