Research on the mechanism of Kaempferol targeting BCL-2 to inhibit lung cancer from the perspective of network pharmacology
10.19405/j.cnki.issn1000-1492.2025.08.002
- VernacularTitle:网络药理学视角下山奈酚靶向 BCL⁃2 抑制肺癌的机制研究
- Author:
Jiandong Chen
1
;
Yingying Lv
2
;
Zheng Xu
2
;
Miao Zhang
2
;
Luyao Liu
2
;
Peng Wang
2
Author Information
1. Dept of Laboratory Medicine , Shu Guang Hospital Afiliated to Shanghai University of Traditional Chinese Medicine , Shanghai 200135
2. Dept of Laboratory Medicine , Shanghai Seventh People ′s Hospital , Shanghai 200135
- Publication Type:Journal Article
- Keywords:
gardenia jasminoides;
kaempferol;
lung cancer;
BCL⁃2;
network pharmacology
- From:
Acta Universitatis Medicinalis Anhui
2025;60(8):1373-1380
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the potential therapeutic mechanisms of kaempferol , an active component in the traditional Chinese medicine gardenia , for lung cancer treatment using a network pharmacology approach .
Methods:The main active ingredients and potential targets of Gardenia jasminoides were obtained through the Tra⁃ditional Chinese Medicine Pharmacology Database and Analysis Platform (TCMSP) , and combined with the lung cancer related target information collected from Gene Cards and OMIM databases , the intersection targets of Garde⁃nia jasminoides and lung cancer treatment were determined by drawing Venn diagrams . Further screening of core targets was conducted through PPI network analysis , and gene ontology (GO) function and Kyoto Encyclopedia of Genes and Genomes ( KEGG) pathway enrichment analysis were performed using the Metascape platform . Auto dock software was used to evaluate the binding affinity between the active ingredients of Gardenia jasminoides and target proteins . In terms of experiments , cell proliferation ability was evaluated through CCK⁃8 assay , cell migration and invasion ability were detected through cell scratch healing assay and Transwell assay , and the expression levels of epithelial mesenchymal transition ( EMT) protein and inflammatory factors were detected by Western blot and RT⁃qPCR .
Results:The active ingredient kaempferol in Gardenia jasminoides exhibited significant binding ability invasion of lung cancer cells . The results of Western blot and RT⁃qPCR further confirmed that kaempferol could promote an increase in E ⁃cadherin , a decrease in N ⁃cadherin and Vimentin , and reduce the expression of inflam⁃matory factors .
Conclusion:The active ingredient of Gardenia jasminoides , kaempferol , inhibits the proliferation ,migration and invasion of lung cancer cells by targeting BCL⁃2 , while reversing EMT progression and suppressing the expression levels of inflammatory cytokines in lung cancer cells , thus preventing lung cancer progression .
- Full text:2026040321573747771网络药理学视角下山奈酚靶向BCL-2抑制肺癌的机制研究_陈建栋.pdf
