Kobophenol A inhibits LPS-induced macrophage M1 polarization via Prdx6
10.19405/j.cnki.issn1000-1492.2025.09.011
- VernacularTitle:Kobophenol A 通过 Prdx6 抑制 LPS 诱导的 巨噬细胞 M1 型极化
- Author:
Tianyu Chen
1
;
Hao Wang
1
;
Jinhong Wang
1
;
Yingjie Zhao
1
;
Renpeng Zhou
1
;
Wei Hu
1
;
Chao Lu
2
Author Information
1. School of Pharmaceutical Sciences，Anhui Medical University，Hefei 230032; Drug Clinical Trial Research Center，The Second Affiliated Hospital of Anhui Medical University，Hefei 230601;
2. School of Pharmaceutical Sciences，Anhui Medical University，Hefei 230032; Clinical Trial Research Center，The First Affiliated Hospital，Anhui University of Science ＆ Technology，Huainan 232007
- Publication Type:Journal Article
- Keywords:
Kobophenol A;
RAW264.7 macrophages;
LPS;
M1 polarization;
Prdx6;
MJ33
- From:
Acta Universitatis Medicinalis Anhui
2025;60(9):1644-1652
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To explore the effects and mechanisms of Kobophenol A ( KPA) on lipopolysaccharide ( LPS) -induced M1 macrophage polarization，and to provide a theoretical basis for the treatment of inflammatory immune diseases and the development of new drugs．
Methods:The M1 macrophage polarization model of RAW264. 7 was established by LPS induction，and the peroxiredoxin 6 ( Prdx6) knockdown model was constructed using the Prdx6 inhibitor MJ33 and Prdx6-siRNA．RAW264. 7 cells，a mouse macrophage cell line，were treated with various concentrations of KPA． Cell viability was assessed using the CCK-8 assay．The expression levels of Prdx6 and M1 macrophage polarization-related proteins，including inducible nitric oxide synthase ( iNOS) and cyclooxygenase-2 ( COX-2) ，were detected by Western blot and immunofluorescence staining．The expression levels of Prdx6 and M1 macrophage polarization-related genes iNOS，interleukin-6 ( IL-6) ，and tumor necrosis factor α ( TNF-α) ，were measured by RT-qPCR. Flow cytometry was employed to detect the expression of cluster of differentiation 86 ( CD86) ，a marker of M1 macrophages．
Results:Compared with the LPS-induced M1 macrophage polarization model ， KPA significantly reversed the morphological changes of M1 macrophage polarization in RAW264. 7 macrophages and decreased the expression of M1 macrophage polarization-related proteins iNOS，COX- 2，CD86 and related genes iNOS，IL-6，TNF-α ( all P＜0. 05) ．In addition，LPS significantly downregulated the expression of Prdx6 in RAW264. 7 macrophages，while KPA upregulated the expression of Prdx6．Moreover，treatment with the Prdx6 inhibitor MJ33 significantly upregulated the expression of iNOS，a marker of M1 macrophage polarization，in RAW264. 7 macrophages，whereas treatment with KPA significantly downregulated the expression of iNOS ( all P＜0. 05) ．
Conclusion:KPA inhibits LPS-induced M1 polarization of RAW264. 7 macrophages by upregulating the expression of Prdx6．
- Full text:2026032719214269079Kobophenol_A通过Prdx6抑制LPS诱导的巨噬细胞M1型极化_陈天宇.pdf
