Effect of vitamin D on NF-κB / CBS-H2 S signaling pathway in rats with chronic sciatic nerve injury
10.19405/j.cnki.issn1000-1492.2025.09.010
- VernacularTitle:维生素 D 对坐骨神经慢性压迫性损伤大鼠 NF-ĸB / CBS-H2 S 信号通路的影响
- Author:
Qin Zhang
1
;
Zhongjin Jiang
1
;
Shengfu Ao
1
Author Information
1. Dept of Anesthesiology，The Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine，Guiyang 550003
- Publication Type:Journal Article
- Keywords:
sciatic nerve chronic injury;
vitamin D;
pain behavior;
the pain threshold;
receptors;
NF-κB/CBS-H_2S signaling pathway
- From:
Acta Universitatis Medicinalis Anhui
2025;60(9):1638-1644
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the ameliorative effect of vitamin D on sciatic nerve chronic compression injury(CCI) rats and its regulatory effect on nuclear factor κB(NF-κB)/cystathione-β-synthase(CBS)-hydrogen sulfide(H2S) system.
Methods:Fifty rats were randomly divided into five groups: control group,model group,vitamin D group,activator group,and activator + vitamin D group,with 10 rats in each group. Except for the control group,the rats in the other groups underwent sciatic nerve ligation to establish the CCI model. The vitamin D group received intraperitoneal injection of 500 mg/kg vitamin D,the activator group received intraperitoneal injection of10 mg/kg NF-κB activator lipopolysaccharide,and the activator + vitamin D group received intraperitoneal injection of 500 mg/kg vitamin D along with 10 mg/kg lipopolysaccharide. The control and model groups received intraperitoneal injection of an equal volume of saline,once per day for 2 weeks. The following parameters were compared among the groups: paw withdraw thermal latency(PWTL),mechanical withdrawal threshold(MWT),serum 25-hydroxyvitamin D3[25(OH)D3]levels,H2S content,calcitonin gene-related peptide(CGRP),prostaglandin E2(PGE2) levels,and the expression of vitamin D receptor(VDR),NF-κBp65,and CBS proteins in the L4-L6 dorsal root ganglia of rats.
Results:Compared with the control group，the model group showed decreased PWTL，MWT，25( OH) D3 levels and VDR protein expression，while H2 S content，CGRP and PGE2 levels，and NF-κBp65 and CBS protein expression significantly increased ( P＜0. 05) ．Compared with the model group，the vi- tamin D group exhibited increased PWTL，MWT，25 ( OH) D3 levels and VDR protein expression ( P ＜0. 05) ， while H2 S content，CGRP and PGE2 levels，and NF-κBp65 and CBS protein expression significantly decreased ( P < 0. 05) ．Compared with the activator group，the activator + Vitamin D group showed increased PWTL，MWT， 25( OH) D3 levels and VDR protein expression，and decreased H2 S content，CGRP and PGE2 levels and decreased NF-κBp65，CBS protein expression ( P＜0. 05) ．Compared with the vitamin D group，the activator + vitamin D group showed decreased PWTL，MWT，25( OH) D3 levels and VDR protein expression，while H2 S content，CGRP and PGE2 levels，and NF-κBp65 and CBS protein expression significantly increased ( P ＜0. 05) ．
Conclusion:Exogenous supplementation of vitamin D can relieve nerve pain and reduce pain sensitivity in CCI rats，possibly by inhibiting NF-κB / CBS-H2 S signaling pathway．
- Full text:2026032716103538415维生素D对坐骨神经慢性压迫性损伤大鼠NF-κB_CBS-H_2S信号通路的影响_张琴.pdf
