Regulation of TGF-β1/JNK signaling pathway in patients with different types of mitral valve diseases complicated by atrial fibrillation
- VernacularTitle:TGF-β1/JNK信号通路在不同类型二尖瓣疾病伴心房颤动患者中的调控作用
- Author:
Chao CHANG
1
;
Bo FU
1
;
Xiaolong ZHU
1
;
Chongjie ZHANG
2
;
Xia ZHAO
3
;
Hong TANG
4
;
Xijun XIAO
5
;
Yunpeng BAI
1
Author Information
1. Department of Cardiovascular Surgery, Tianjin Chest Hospital, Tianjin University, Tianjin, 300222, P. R. China
2. Department of Immunology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, 610041, P. R. China
3. Laboratory of Gynecological Oncology Biotherapy, West China Second University Hospital, Sichuan University, Chengdu, 610041, P. R. China
4. Department of Cardiology, West China Hospital, Sichuan University, Chengdu, 610041, P. R. China
5. Department of Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu, 610041, P. R. China
- Publication Type:Journal Article
- Keywords:
Atrial fibrillation;
mitral valve disease;
TGF-β1/JNK signaling pathway;
apoptosis;
atrial structural remodeling;
mitral regurgitation;
mitral stenosis;
hemodynamics
- From:
Chinese Journal of Clinical Thoracic and Cardiovascular Surgery
2026;33(02):291-299
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the regulatory mechanism of transforming growth factor-β1 (TGF-β1) in different types of mitral valvular disease (MVD) with atrial fibrillation (AF). Methods From August 2011 to August 2012, patients with moderate to severe MVD accompanied by AF who required mitral valve replacement at the Department of Cardiovascular Surgery, West China Hospital, Sichuan University, were included. Based on echocardiographic results, patients were divided into two groups: a mitral regurgitation (MR) with AF (MR-AF) group and a mitral stenosis (MS) with AF (MS-AF) group. Left atrial tissue samples were collected during surgery. Techniques such as enzyme-linked immunosorbent assay, real-time fluorescence quantitative polymerase chain reaction, immunohistochemistry, and Western blotting were used to detect key molecules in the TGF-β1/JNK pathway. Results Sixteen patients were enrolled. There were 8 patients in the MR-AF group, including 5 males and 3 females, with an average age of (41.38±11.19) years; and 8 patients in the MS-AF group, including 6 males and 2 females, with an average age of (43.12±5.30) years. The left atrial volume load was higher in MR-AF patients, while the left atrial pressure load was higher in MS-AF patients. In MS-AF patients, the relative expression levels of MAPK9, JUN, CASP3, BAX, and BCL2 mRNA in left atrial tissues were significantly upregulated. The serum TGF-β1 protein level and the relative expression levels of p-JNK, p-c-Jun, and Caspase-3 proteins in the left atrial tissues of the MR-AF group were higher. Myocardial cell damage was more severe in the MS-AF group, and the protein expression level of Bcl-2 was higher. Conclusion Different MVD have distinct hemodynamic characteristics. The myocardium of the left atrium in MR-AF patients is more prone to apoptosis, possibly through the activation of the TGF-β1/JNK signaling pathway.
