Application of Poisson distribution method in determination of herpes simplex virus 2 viral titer
10.13200/j.cnki.cjb.004662
- VernacularTitle:泊松分布法在Ⅱ型单纯疱疹病毒感染滴度测定中的应用
- Author:
Guangyu WANG
1
Author Information
1. National Key Laboratory of Drug Regulatory Science, National Institutes for Food and Drug Control, Beijing 100050, China
- Publication Type:Journal Article
- Keywords:
Oncolytic viruses(OVs);
Herpes simplex virus(HSV);
Viral titer;
Poisson distribution method;
CCID_(50) method;
Quality control
- From:
Chinese Journal of Biologicals
2026;39(03):326-330
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo develop an accurate quantitative method of herpes simplex virus 2(HSV2) oncolytic viruses(OVs)viral titer based on Poisson distribution principle, aiming at the limitation of high variability and complicated operation of traditional half cell culture infectious dose method(CCID_(50)), so as to improve the accuracy and stability of quality control.MethodsA robust detection system was established by optimizing Vero cell culture conditions(serum concentration and incubation time after virus infection). HSV2 titer was determined simultaneously by traditional CCID_(50)method and new Poisson distribution method(maximum likelihood estimation using negative well data based on high-density 96-well microplate).ResultsThe mean virus titer measured by CCID_(50)method was 1. 75 × 10~6 CCID_(50)/mL, with relative standard deviation(RSD) of 36. 62%, while that measured by Poisson distribution method was 7. 94 × 10~5 ifu/mL, with significantly reduced variability(RSD of 12. 59%).ConclusionPoisson distribution method significantly improves the accuracy and efficiency of virus titer determination by fully integrating microplate data to reduce random error, and provides a standardized and reliable solution for OVs quality evaluation, which has broad clinical application prospects.
