Study on the extraction, separation and purification process of Actinoside E
10.12206/j.issn.2097-2024.202407001
- VernacularTitle:对萼猕猴桃苷E提取分离纯化工艺的研究
- Author:
Fangliang QIAO
1
;
Yiping JIANG
1
;
Tianshuang XIA
1
;
Aijun LIU
2
;
Kai ZHAO
3
;
Hailiang XIN
1
Author Information
1. School of Pharmacy, Naval Medical University, Shanghai 200433, China.
2. Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China.
3. Hebei Kingsci Pharmaceutical Technology Co., LTD, Shijiazhuang 050035, China.
- Publication Type:Originalarticles
- Keywords:
Actinoside E;
extraction;
separation and purification;
chromatography
- From:
Journal of Pharmaceutical Practice and Service
2026;44(3):137-143
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize the extraction, separation and purification process of Actinoside E. Methods Single factor experiment combined with orthogonal test was used to determine the optimal extraction process of Actinoside E using its content as an index. The extracts were separated and purified by optimizing the chromatographic conditions of macroporous resin, silica gel and ODS column. Results 25 times amount of 55% ethanol with heating reflux at 95℃ for one hour were used as the optimal extraction process of Actinoside E. The optimum separation and purification process was as follows: D101 macroporous resin column was eluted with 7 BV of 50% ethanol, silica gel column was eluted with 5 BV of ethyl acetate-ethanol（10∶1）and 50% methanol eluted fraction was purified repeatedly by ODS column to obtain Actinoside E. The transfer rate of Actinoside E in the whole process was 53.70%, the yield was 0.35%, and the purity was 99.9%. Conclusion The process is stable and viable, which can provide material foundation for the development and utilization of Actinoside E.
