Construction of glucose oxidase–loaded nanogels and its inhibition effect on the Warburg effect in glioma cells
10.12206/j.issn.2097-2024.202511030
- VernacularTitle:载葡萄糖氧化酶纳米凝胶的构建及对胶质瘤细胞Warburg效应的抑制
- Author:
Wenbo ZHOU
1
;
Weilin LI
1
;
Wuting DAI
1
;
Ruiyao LIU
1
;
Yuan YU
1
Author Information
1. School of Pharmacy, Naval Medical University, Shanghai 200433, China.
- Publication Type:Originalarticles
- Keywords:
glioblastoma;
nanogels;
glucose oxidase;
Warburg effect
- From:
Journal of Pharmaceutical Practice and Service
2026;44(3):132-136
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct glucose oxidase（GOx）–loaded nanogels （GONGs）, optimize their formulation, and evaluate their capacity to inhibit the Warburg effect in glioma cells. Methods A responsive polymer （HAM） was synthesized and used to self-assemble GONGs, which were then characterized. Encapsulation efficiency and drug loading were determined using fluorescence spectrophotometry. Biocompatibility was tested by measuring cytotoxicity and hemolytic activity. Western blotting was used to evaluate the effects of GONGs on the expression of proteins associated with the Warburg phenotype and oxidative damage in glioma cells. Results GONGs prepared at a drug–to–polymer ratio of 1∶10 exhibited a particle size of 140.3 nm and a zeta potential of −27.2 mV. Compared with free GOx, GONGs markedly reduced cytotoxicity, increased the IC50 in hUVEC cells from 2.150 nmol/L to 74.86 nmol/L, and significantly decreased hemolysis. At a GOx concentration of 2 nmol/L, GONGs effectively downregulated glycolysis-related proteins, such as HK2 and LDHA, and inhibited glutamine metabolism in glioma cells. Conclusion GONGs exhibited high GOx loading capacity, significantly reduced GOx-induced cytotoxicity, inhibited the Warburg effect in glioma cells and induced oxidative damage.
