Targeted fluorescent imaging probes for detecting the spatial distribution of VEGF in the retinas of rats with radiation retinopathy
10.3980/j.issn.1672-5123.2026.4.03
- VernacularTitle:靶向荧光成像探针检测VEGF在RR大鼠视网膜的空间分布
- Author:
Yunhe DING
1
;
Bin WANG
1
;
Feng LIU
1
;
Zhiyang ZHANG
1
;
Haibei DONG
1
;
Wenwen GUO
1
;
Haitao YIN
1
Author Information
1. Cancer Center, Xuzhou First People's Hospital, Xuzhou 221100, Jiangsu Province, China
- Publication Type:Journal Article
- Keywords:
radiation retinopathy(RR);
vascular endothelial growth factor(VEGF);
ranibizumab;
molecular fluorescence imaging
- From:
International Eye Science
2026;26(4):567-572
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To detect the distribution and expression of vascular endothelial growth factor(VEGF)in radiation retinopathy(RR)through fluorescence targeted imaging.METHODS:Covalent binding of fluorescein FITC with VEGF antibody ranibizumab to prepare targeted fluorescent imaging probe ranibizumab-FITC. SD rats were randomly divided into three groups based on the principle of weight balance: a normal control group(Con group), a low-dose radiation group(10 Gy group), and a high-dose radiation group(30 Gy group). Medical linear accelerators and lead blocks were used to locally irradiate the rat eyeballs for modeling. Western blot and qRT-PCR were used to detect the expression levels of VEGF-A in each group and to screen for appropriate modeling dose. The inverted fluorescence microscope and the confocal microscope were used to observe the distribution of VEGF and imaging probes in the retinas of control and RR model group rats, and to verify the effectiveness of targeted probes.RESULTS:The expression level of VEGF-A in the retina of rats in the high-dose radiation group(30 Gy group)was higher than that in the normal control group(Con group). In early RR, VEGF expression was observed to be associated with microaneurysms and abnormal microvessels in the retina. VEGF accumulation was observed at the site of capillary wall damage. When retinal capillary endothelial damage occurred, targeted probes gathered on the outer surface of the vessel wall.CONCLUSION:The expression level of VEGF in the retina of RR model rats is elevated, and fluorescent targeted molecular imaging probes can detect the spatial distribution of VEGF at the microvascular lesions in the retina of RR rats.
