Establishment and identification of hepatocyte-specific Tmem121 knockout mouse model
10.19405/j.cnki.issn1000-1492.2025.09.004
- VernacularTitle:Tmem121 肝细胞特异性敲除小鼠模型的建立与鉴定
- Author:
Yue Wang
1
;
Guoliang He
1
;
Lanyu Li
2
;
Qian Wu
3
;
Junmei Zhou
4
Author Information
1. Guangxi Key Laboratory of Molecular Medicine in Liver Injury and Repair，The Affiliated Hospital of Guilin Medical University，Guilin 541001
2. Laboratory Animal Center，Guilin Medical University，Guilin 541001
3. Clinical Medical College，Guilin Medical University，Guilin 541001
4. Guangxi Key Laboratory of Molecular Medicine in Liver Injury and Repair，Guangxi Health Commission Key Laboratory of Basic Research in Sphingolipid Metabolism Related Diseases，Laboratory of Hepatobiliary and Pancreatic Surgery，The Affiliated Hospital of Guilin Medical University，Guilin 541001;China-USA Lipids in Health and Disease Research Center，Guilin Medical University，Guilin 541001
- Publication Type:Journal Article
- Keywords:
transmembrane protein 121;
specific knockout;
Cre/LoxP system;
hepatocytes;
genotype identification;
primary hepatocyte isolation
- From:
Acta Universitatis Medicinalis Anhui
2025;60(9):1591-1598
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish and identify hepatocyte-specific transmembrane protein 121 ( Tmem121 ) knockout mice．
Methods:The hepatocyte-specific Tmem121 knockout mice ( Tmem121flox / flox / Cre，Tmem121ΔHep) were obtained by crossbreeding of Tmem121flox / + / Cre and Tmem121flox / flox mice，which were generated using the CRISPR / Cas9 and Cre / Loxp systems．The genotype was verified by PCR using genomic DNA extracted from mouse tails as template．The growth，reproduction and organ development of both control and knockout mice were ob- served and analyzed．PCR and Western blot methods were performed to assess the knockout efficiency of Tmem121 in mouse primary hepatocytes．CellMaskTM Deep Red plasma membrane staining was employed to compare the mor- phological differences in primary hepatocytes between control and knockout mice．
Results:Tmem121flox / flox / Cre mice were successfully obtained according to genotype identification analysis，and there were no significant differ- ences between control and knockout mice in body mass，reproductive ability，growth and development of liver．The specific knockout of Tmem121 gene in primary hepatocytes did not significantly affect the morphological structure or pathological characteristics of liver tissue．However，compared to the control group，the levels of Tmem121 mRNA and protein in the primary hepatocytes of the knockout group were significantly reduced ( P ＜0. 01) ．CellMaskTM Deep Red plasma membrane staining indicated that the proportion of binucleated hepatocytes in Tmem121-deficient mice significantly increased ( P＜0. 05) ，while the cell area was significantly reduced ( P＜0. 001) ．
Conclusion:Hepatocyte-specific Tmem121 knockout mice are successfully constructed，which provides an animal model for further exploration of the function and mechanism of Tmem121 gene in liver diseases．
- Full text:2026031611090989133Tmem121肝细胞特异性敲除小鼠模型的建立与鉴定_王月.pdf
