Preliminary application of histological evaluation of donor pancreas biopsy tissue in simultaneous pancreas-kidney transplantation
10.12464/j.issn.1674-7445.2025302
- VernacularTitle:供胰腺穿刺活检组织学评估在胰肾联合移植中的初步应用
- Author:
Jiao WAN
1
;
Hui GUO
2
;
Jiali FANG
1
;
Guanghui LI
1
;
Luhao LIU
1
;
Yunyi XIONG
1
;
Wei YIN
1
;
Tong YANG
1
;
Junjie MA
1
;
Zheng CHEN
1
Author Information
1. Department of Organ Transplantation, the Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 511447, China.
2. null.
- Publication Type:OriginalArticle
- Keywords:
Simultaneous pancreas-kidney transplantation;
Donor pancrea;
Quality assessment;
Puncture;
Biopsy;
Pathological assessment;
Fat infiltration;
Fibrosis
- From:
Organ Transplantation
2026;17(2):250-256
- CountryChina
- Language:Chinese
-
Abstract:
Objective To preliminarily investigate the safety and efficacy of donor pancreas needle biopsy in simultaneous pancreas-kidney transplantation. Methods Clinical data of 7 cases undergoing donor pancreas biopsy were collected retrospectively. All cases underwent donor pancreas biopsy before or during simultaneous pancreas-kidney transplantation. Frozen section or paraffin sectioning techniques were used for tissue preparation, and hematoxylin-eosin and Masson staining were performed to histologically evaluate the donor pancreas. The quality of donor pancreas was comprehensively assessed by combining histological findings with the donor's clinical data. Postoperative follow-up data of 5 simultaneous pancreas-kidney transplant recipients were collected to summarize the safety of donor pancreas biopsy and the prognosis of transplant recipients. Results The 7 pancreas donors were aged 28 to 62 years, with a body mass index ranging from 20.76 to 27.68 kg/m2. Liver ultrasound indicated fatty liver in 3 cases, while pancreatic ultrasound did not reveal any significant abnormalities. Among them, biopsy was performed on 2 donors after completion of pancreatic procurement and processing, and the frozen section histology showed moderate acute pancreatitis changes (edema of acinar cells, necrosis and inflammatory cell infiltration). Combined with a serum amylase level elevated more than 3 times the upper limit of normal value, these two donor pancreases were finally discarded. The remaining 5 cases underwent biopsy immediately after pancreatic vascular anastomosis during simultaneous pancreas-kidney transplantation, and histological evaluation was performed on paraffin-embedded sections. No biopsy-related complications (such as bleeding, pancreatic fistula, etc.) occurred after transplantation. One recipient died of severe infection 2 months after transplantation, while the other 4 recipients were followed up for more than 5 years, with well-functioning transplant kidneys and pancreases. Conclusions Donor pancreas biopsy is relatively safe, and the risk of biopsy-related complications after transplantation is controllable. Comprehensive assessment of donor pancreas quality by combining histological evaluation with the donor's clinical indicators is conducive to improving the accuracy of donor pancreas selection and organ utilization.
