Inhibitory effect and mechanism of asiaticoside on hypertrophic scar formation in rabbit ears
- VernacularTitle:积雪草苷对兔耳增生性瘢痕形成的抑制作用及机制
- Author:
Qiai LI
1
;
Wenjiao ZHOU
1
;
Bingcheng XU
2
Author Information
1. Dept. of Dermatology，Wuhan Wuchang Hospital，Wuhan 430064，China
2. Dept. of Geriatrics，Wuhan Wuchang Hospital，Wuhan 430064，China
- Publication Type:Journal Article
- Keywords:
asiaticoside;
hypertrophic scar;
Wnt/β-catenin
- From:
China Pharmacy
2026;37(5):607-612
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore the inhibitory effect and its mechanism of asiaticoside on hypertrophic scar formation in rabbit ears based on the Wnt/ β -catenin pathway. METHODS The hypertrophic scar model in rabbit ears was established. Model rabbits were divided into the model group （normal saline）， asiaticoside low-dose group （12 mg/kg）， asiaticoside high-dose group （24 mg/kg）， and positive control group （tri amcinolone acetonide acetate 40 mg/kg）； additionally， a non-modeled control group （normal saline） was set up. There were 8 rabbits in each group. Rabbits in each group were injected with the corresponding drug solution/normal saline at the base within the scar area once daily for 28 consecutive days. After the last medication， the appearance of hypertrophic scars in rabbit ears was observed， and the hypertrophic scar index was measured； the levels of inflammatory factors [interleukin-6 （IL-6） and IL-10 ] in hypertrophic scar tissue of rabbits were determined； histopathological morphology of hypertrophic scar tissues in rabbit ears was observed， and the protein expression levels of collagen type Ⅰ （COLⅠ）， COLⅢ， Wnt4 and β -catenin in the hypertrophic scar tissues were detected. RESULTS Compared with control group， in the model group， the symptoms of hypertrophic scars were observed， the stratum corneum was thicker， and there was abnormal accumulation and disorganized arrangement of collagen. The hypertrophic scar index， IL-6 level， fibroblast density， area density of collagen fibers， mean optical density values of COLⅠ and COLⅢ， as well as the expression levels of Wnt4 and β -catenin proteins， were all significantly elevated （ P ＜0.05）， while the level of IL-10 was significantly decreased （ P ＜0.05）. Compared with the model group， the hypertrophic scars in asiaticoside low- and high-dose groups were significantly softened， with a thinned stratum corneum， and the above quantitative indicators were all significantly reversed （ P ＜0.05）. CONCLUSIONS Asiaticoside can inhibit the formation of hypertrophic scars in rabbit ears and alleviate the inflammatory response in scar tissue. Its mechanism of action may be related to the inhibition of activation of the Wnt/ β -catenin pathway.
