Effect and Mechanism of Exogenous NO in Secondary Metabolism in Scutellaria baicalensis
10.13422/j.cnki.syfjx.20251318
- VernacularTitle:外源NO对黄芩次生代谢影响及机制分析
- Author:
Kai ZHAO
1
;
Wei MA
1
;
Weili LIU
1
;
Zhihong LOU
2
;
Xiangcai MENG
1
Author Information
1. Heilongjiang University of Chinese Medicine， Harbin 150040，China
2. Heilongjiang Institute for Drug Control， Harbin 150088，China
- Publication Type:Journal Article
- Keywords:
Scutellariae Radix;
stress;
reactive oxygen species;
secondary metabolites;
quality of medicinal materials
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2026;32(1):250-261
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effects of exogenous nitric oxide （NO） on the accumulation and quality formation mechanism of flavonoids in Scutellariae Radix. MethodsFresh roots of Scutellaria baicalensis were treated with sodium nitroprusside （SNP） solutions at concentrations of 0.0， 7.5， and 20 mmol·L-1， respectively. Kits and supporting reaction systems were used to determine the following indicators of samples in each group， including （1） reactive oxygen species： changes in the content of nitric oxide （NO）， superoxide anion （O2-
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·）， and hydrogen peroxide （H2O2）， （2） lipid oxidation products： changes in the content of malondialdehyde （MDA）， （3） antioxidant enzymes： changes in the activity of superoxide dismutase （SOD）， catalase （CAT）， and peroxidase （POD）， and （4） metabolic key enzymes and metabolic intermediates： Changes in the activity of phenylalanine ammonia-lyase （PAL） and the content of 1，3-diphosphoglycerate （1，3-DPG）. High-performance liquid chromatography （HPLC） was used to determine the changes in the content of secondary metabolites in flavonoids such as baicalin， wogonoside， baicalein， and wogonin in each group. ResultsCompared with that on day 0， during the treatment with 20 mmol·L-1 SNP solution， the content of NO， O2-![]()
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·， H2O2， and MDA in fresh roots of Scutellaria baicalensis increased significantly （P<0.01）， by 138.7%， 75.7%， 292.9%， and 140.3% respectively. The activity of SOD， CAT， and POD increased by 100.7%， 41.4%， and 70.4% respectively （P<0.01）， and that of PAL increased by 111.1% （P<0.01）. The content of the secondary metabolites baicalin and wogonoside remained basically unchanged， while that of baicalein and wogonin increased by 401.3% and 457.9% respectively （P<0.01）. The content of 1，3-DPG increased by 165.0% （P<0.01）. In the 7.5 mmol·L-1 SNP solution treatment group， the stress degree was relatively mild. Compared with that on day 0， the content of NO， O2-![]()
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·， H2O2， and MDA increased by 76.2%， 57.7%， 221.4%， and 99.7% respectively （P<0.01）. The activity of antioxidant enzymes SOD， CAT， and POD increased by 79.5%， 55.6%， and 51.9% respectively （P<0.01）， and that of PAL increased by 69.0% （P<0.01）. The content of baicalein and wogonin increased by 204.7% and 272.2% respectively （P<0.01）， and that of 1，3-DPG increased by 128.0% （P<0.01）. The 20 mmol·L-1 SNP group showed better performance than the 7.5 mmol·L-1 SNP group. ConclusionSNP can simulate the physiological state of Scutellaria baicalensis under stress conditions， activate the antioxidant protection mechanism， promote the biosynthesis of flavonoids in Scutellariae Radix， and significantly improve the quality of medicinal materials in Scutellariae Radix.
