Metabolomics Reveals Mechanism of Jatrorrhizine in Treating Ulcerative Colitis in Mice
10.13422/j.cnki.syfjx.20241619
- VernacularTitle:基于代谢组学探究药根碱治疗小鼠溃疡性结肠炎的作用机制
- Author:
Shengqi NIU
1
;
Liwei LANG
1
;
Xing LI
1
;
Haotian LI
1
;
Shizhang WEI
1
;
Manyi JING
1
;
Yanling ZHAO
1
Author Information
1. The Fifth Medical Center of Chinese PLA General Hospital，Beijing 100039，China
- Publication Type:Journal Article
- Keywords:
jatrorrhizine;
ulcerative colitis;
metabolomics;
dextran sulfate sodium
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2026;32(1):211-218
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effects of jatrorrhizine on endogenous metabolites and metabolic pathways in the mouse model of ulcerative colitis. MethodsThirty male C57BL/6J mice were randomly divided into the normal group， the model group， the low-dose and high-dose jatrorrhizine groups （0.04， 0.16 g·kg-1）， and the mesalazine group （0.52 g·kg-1）The mouse model of ulcerative colitis was established with 3% dextran sulfate sodium （DSS） and treated with different doses of jatrorrhizine by gavage. The changes in body weight， colon length， disease activity index （DAI）， and colonic histopathology were analyzed to evaluate the therapeutic effects of jatrorrhizine. UPLC-Q-TOF/MS was employed to determine the serum and fecal levels of metabolites in mice. Metabolomics methods were used to screen the differential metabolites， on the basis of which the potential therapeutic mechanism of jatrorrhizine on DSS-induced ulcerative colitis in mice was investigated. ResultsAfter intervention with jatrorrhizine， the model mice showed significantly decreased DAI（P<0.05，P<0.01）， recovered colon length，（P<0.05，P<0.01） and alleviated histopathology of the colon. The metabolomics study screened out 13 differential metabolites in the serum and 8 differential metabolites in the feces. The pathway enrichment analysis predicted three potential metabolic pathways： Biosynthesis of unsaturated fatty acids， phenylalanine， tyrosine and tryptophan biosynthesis， and phenylalanine metabolism. ConclusionJatrorrhizine may treat ulcerative colitis by regulating the biosynthesis and metabolism of amino acids and the synthesis of unsaturated fatty acids.
