Csf1rCre -mediated yellow fluorescent protein can effectively label tissue-resident macrophages in various tissues
10.19405/j.cnki.issn1000-1492.2025.09.001
- VernacularTitle:Csf1rCre 介导的黄色荧光素蛋白可有效标记 各组织定居巨噬细胞
- Author:
Xiaoyu Li
1
;
Dianyuan Zhao
2
;
Shengquan Zhang
1
;
Jing Yang
3
;
Li Tang
3
Author Information
1. Dept of Biochemistry，School of Basic Medicine，Anhui Medical University，Hefei 230032
2. Institute of Lifeomics， Academy of Military Medical Sciences，Academy of Military Sciences，Beijing 102206; State Key Laboratory of Proteomics，Beijing Proteome Research Center，National Center for Protein Science，Beijing 102206
3. Dept of Biochemistry，School of Basic Medicine，Anhui Medical University，Hefei 230032;Institute of Lifeomics， Academy of Military Medical Sciences，Academy of Military Sciences，Beijing 102206; State Key Laboratory of Proteomics，Beijing Proteome Research Center，National Center for Protein Science，Beijing 102206
- Publication Type:Journal Article
- Keywords:
Csf1r~(Cre) recombinase;
reporter gene mice;
flow cytometry;
tissue-resident macrophage;
monocytes;
cell labeling efficiency
- From:
Acta Universitatis Medicinalis Anhui
2025;60(9):1567-1573
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To construct Csf1rCreRosa26YFPmice,and to detect the efficiency of Csf1rCre-mediated yellow fluorescent protein(YFP) labeling monocytes and tissue-resident macrophages in different tissues.
Methods:The Rosa26YFPmutant mice had a loxP-flanked STOP sequence,followed by a yellow fluorescent protein gene(YFP)inserted into the Rosa26 locus. When crossed with mice expressing the Csf1rCrerecombinase,the STOP sequence was deleted,and yellow fluorescent protein(YFP) expression was observed in the tissues of double-mutant offspring(Csf1rCreRosa26YFP). Csf1rCremice were mated with Rosa26YFPmice,and genotype of Csf1rCreRosa26YFPmice were identified by PCR. Blood and bone marrow monocytes,liver macrophages,kidney macrophages,alveolar macrophages and spleen macrophages of adult Csf1rCreRosa26YFPmice were isolated,labeled with flow cytometry antibodies,and the recombination efficiency of Csf1rCre-mediated YFP labeling tissue macrophages was analyzed by flow cytometry.
Results:In Csf1rCreRosa26YFPreporter mice,the median percentage of YFP + was 93. 25% in renal macrophages,92. 45% in liver macrophages,and 91. 10% in spleen macrophages. The percentage of YFP +was 94. 70% in alveolar macrophages,98. 20% in blood monocytes,and 93. 90% in bone marrow monocytes.
Conclusion:Csf1rCrecan be used to trace tissue-resident macrophages as well as bone marrow and blood monocytes. At the same time,Csf1rCrecan target these cells to prepare conditional gene knockout mice.
- Full text:2026031100081022153Csf1r~(Cre)介导的黄色荧光素蛋白可有效标记各组织定居巨噬细胞_李晓宇.pdf
