Application of metagene next-generation sequencing of alveolar lavage fluid in the detection of pathogenic bacteria of pulmonary infection
10.19405/j.cnki.issn1000-1492.2025.10.018
- VernacularTitle:肺泡灌洗液宏基因组 二代测序在检测肺部感染病原菌中的应用
- Author:
He Zhang
1
;
Xinyue Luo
1
;
Xin Heng
1
;
Yun Zhang
1
;
Songping Wang
1
;
Jun Deng
1
Author Information
1. Dept of Respiratory and Critical Care Medicine , Afiliated Hospital of Southwest Medical University, Luzhou 646000
- Publication Type:Journal Article
- Keywords:
pulmonary infection;
metagenomic second generation sequencing;
mNGS;
conventional detection;
alveolar lavage fluid;
underlying medical conditions;
pathogen
- From:
Acta Universitatis Medicinalis Anhui
2025;60(10):1917-1923,1931
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the value of metagene next⁃generation sequencing ( mNGS) in the detection of pathogens in patients with pulmonary infection.
Methods:A retrospective analysis was performed on clinical data from 434 patients with pulmonary infections admitted over the past four years. Based on the presence of underlying comorbidities , patients were divided into underlying disease group (n = 262) and non⁃underlying disease group (n = 172) . Pathogen detection was conducted using both mNGS and conventional tests. Clinical and laboratory parameters , radiographic findings , and pathogen detection results were systematically analyzed. The diagnostic performance of the two methods in identifying causative pathogens of pulmonary infections was compared.
Results:The positive rate of mNGS in 434 patients was higher than that of conventional tests , and the difference was statisti⁃cally significant (P < 0. 05) . The efficacy of mNGS in detecting bacteria and viruses was significantly higher than that of conventional tests , and the difference was statistically significant (P < 0. 05) . Although the fungal detection rate of mNGS was higher than that of conventional tests , the difference was not statistically significant. Among them , the detection rates of Mycobacterium tuberculosis , Mycoplasma pneumoniae , Haemophilus influenzae , Strepto⁃ coccus pneumoniae , Streptococcus constellation , Staphylococcus aureus and Aspergillus fumigatus were significantly higher than those of conventional tests , and the difference was statistically significant (P < 0. 05) . Subgroup analy- sis showed that the proportion of males , hospital stay , smoking prevalence and average age in the underlying dis- ease group were higher than those in the non-underlying disease group , and the difference was statistically signifi- cant (P < 0. 05) , while there were no significant differences in antibiotic use and endotracheal intubation rate be- tween the two groups. The most common pathogens detected by mNGS in the underlying disease group were Myco⁃ bacterium tuberculosis , Haemophilus influenzae , Streptococcus pneumoniae , Pseudomonas aeruginosa , human herpes⁃ virus type 4 and Aspergillus fumigatus , while the most common pathogens in the non-underlying disease group were Mycobacterium tuberculosis , Haemophilus influenzae , Streptococcus pneumoniae , Mycoplasma pneumoniae and Kleb⁃ siella pneumoniae. The positive rate of mNGS in the two groups was significantly higher than that of conventional tests , and the difference was statistically significant (P < 0. 05) , while the difference in the positive rate of mNGS between the two groups was not statistically significant.
Conclusion:mNGS has significant advantages over con- ventional tests of pathogen in lung infection , and is less affected by underlying diseases , which can provide an etio- logical basis for lung infection.
- Full text:2026031022485229745肺泡灌洗液宏基因组二代测序在检测肺部感染病原菌中的应用_张鹤.pdf
