Peptidylarginine deiminase 4 regulates IL-8 secretion involves in the pathogenesis of systemic lupus erythematosus
10.19405/j.cnki.issn1000-1492.2025.10.013
- VernacularTitle:肽基精氨酸脱亚胺酶 4 调控 IL⁃8 分泌参与 系统性红斑狼疮发病的研究
- Author:
Jie Ma
1
;
Xiaoqing Wang
1
;
Tingdong Zhou
1
;
Ying Luo
1
;
Qiang Zhou
1
Author Information
1. Dept of Clinical Laboratory, The Second Afiliated Hospital of Anhui Medical University, Hefei 230601
- Publication Type:Journal Article
- Keywords:
systemic lupus erythematosus;
interleukin 8;
peptidylarginine deiminase 4;
inflammation;
neutrophils;
cytokine
- From:
Acta Universitatis Medicinalis Anhui
2025;60(10):1874-1878,1886
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the regulation of interleukin 8(IL-8) secretion by peptidylarginine deiminase 4(PADI4) and its effect on the pathogenesis of systemic lupus erythematosus(SLE).
Methods:qRT-PCR was used to compare the expression levels of IL-8 in neutrophils between healthy controls(HC) and SLE patients. ELISA was used to detect IL-8 secretion levels in the serum of HC and SLE patients, the correlation between serum IL-8 and SLE related serological indicators in lupus patients was analyzed. ELISA was used to detect the effect of HC orSLE serum on IL-8 secretion by neutrophils. Using PADI4-specific inhibitor GSK484 in primary neutrophils, or in PADI4-knockdown neutrophil-like HL-60 cells(dHL-60), IL-8 stimulated by N-formyl-met-leu-phe(fMLP) or immune complexes(ICs) was detected.
Results:Compared with HC, IL-8 was significantly higher expressed in neutrophils of SLE patients. Serum IL-8 levels significantly increased in lupus patients and were positively correlated with serum IgM levels. Serum from SLE patients induced neutrophils to secrete more IL-8. PADI4 inhibitor could upregulate the production of IL-8 in neutrophils. In dHL-60 cells, knockdown of PADI4 led to a significant increase in IL-8 secretion.
Conclusion:The proinflammatory cytokine IL-8 is highly expressed in neutrophils and serum of SLE patients, regulated by PADI4 and correlated with lupus serological indicators. IL-8 plays a role in the development of SLE through inflammatory responses, and PADI4/IL-8 provides new thinking for SLE monitoring and therapy.
- Full text:2026030923510374053肽基精氨酸脱亚胺酶4调控IL-8分泌参与系统性红斑狼疮发病的研究_马洁.pdf
