Aloperine inhibits the malignant progression of gastric cancer cells by modulating the Hippo pathway
10.19405/j.cnki.issn1000-1492.2025.10.011
- VernacularTitle:苦豆碱通过调节 Hippo 通路抑制胃癌细胞恶性进程
- Author:
Qiqi Tan
1
;
Bingxue Lan
1
;
Junhua Zhang
2
;
Hai Huang
1
Author Information
1. Center for Clinical Laboratories , The Afiliated Hospital of Guizhou Medical University, Guiyang 550004 ; School of Clinical Laboratory Science , Guizhou Medical University, Guiyang 550004
2. Dept of Blood Transfusion , The Third Xiangya Hospital of Central South University, Changsha 410013
- Publication Type:Journal Article
- Keywords:
aloperine;
gastric cancer;
Hippo signaling pathway;
HGC-27 cells;
AGS cells
- From:
Acta Universitatis Medicinalis Anhui
2025;60(10):1857-1865
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of aloperine (Alo) on the proliferation , migration and invasion of gastric cancer cells.
Methods:Human gastric cancer cell lines HGC⁃27 and AGS were treated with 0 , 100 , and tion. Flow cytometry was used to detect apoptosis. Scratch and Transwell migration assays were used to detect cell migration. Transwell invasion assay was used to detect cell invasion. Western blot was used to detect the expression of proteins related to proliferation , apoptosis , migration , invasion and Hippo pathway.
Results:Compared with the control group , the cell viability , number of colony formation , cell migration and invasion were significantly reduced after 100 and 200 μmol/L Alo treatment , and the apoptosis rate significantly increased (P < 0. 05) . Additionally , the expressions of proliferating cell nuclear antigen (PCNA) , B -cell lymphoma/leukemia⁃2 protein (Bcl⁃2) , neural cadherin ( N⁃cadherin) , Vimentin , and transcriptional co⁃activator with PDZ⁃binding motif ( TAZ)were significantly reduced , whereas the expressions of Bcl⁃2 ⁃associated X protein (Bax) , phosphorylated yes⁃asso⁃ciated protein (p⁃YAP) , and large tumor suppressor 1/2 (LATS1/2) significantly increased (P < 0. 05) .
Conclusion:Alo inhibits the proliferation , migration and invasion of gastric cancer cells HGC⁃27 and AGS by regulating the Hippo signaling pathway.
- Full text:2026030822012637523苦豆碱通过调节Hippo通路抑制胃癌细胞恶性进程_谭琪琪.pdf
