Ginkgo biloba extract down-regulates TLR4/NLRP3 signaling to protect airway inflammation in COPD rats
10.19405/j.cnki.issn1000-1492.2025.10.008
- VernacularTitle:银杏叶提取物下调 TLR4/NLRP3 信号保护 COPD 大鼠气道炎症损伤
- Author:
Ying Pan
1
;
Xueni Mo
1
;
Gerui Wang
1
;
Yuqing Feng
2
;
Fang Xie
2
;
Meiling Mao
1
;
Tingting Wei
1
;
Jing Xiang
3
;
Lianjian Huang
1
;
Fanbo Wei
1
;
Yibao Yang
2
Author Information
1. Graduate School of Guangxi University of Chinese Medicine ,Nanning 530011
2. Dept of Critical Care Medicine Ruikang Hospital Afiliated to Guangxi University of Chinese Medicine ,Nanning 530023
3. Graduate School of Guangxi University of Chinese Medicine ,Nanning 530011Graduate School of Guangxi University of Chinese Medicine ,Nanning 530011
- Publication Type:Journal Article
- Keywords:
ginkgo biloba extract;
chronic obstructive pulmonary disease;
TLR4/NLRP3 signaling pathway;
inflammation of the airways;
airway remodeling;
animal experiment
- From:
Acta Universitatis Medicinalis Anhui
2025;60(10):1833-1838
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the regulatory effects of ginkgo biloba extract on airway inflammatory injury and Toll⁃like receptor 4(TLR4)/nucleotide⁃binding oligomerization domain⁃containing 3(NLRP3) pathway in rats with vided into four groups : the normal control group ,
Methods:Thirty⁃six male SD rats were selected and randomly divided into four groups : the normal control group , the model group , the prednisone treatment group , and the ginkgo biloba extract treatment group , with 9 rats in each group. Except for the normal control group , the COPD rat mod⁃els in the other groups was constructed by intratracheal instillation of lipopolysaccharide (LPS) combined with ciga⁃rette smoke exposure. After successful modeling , the rats were continuously administered drugs for 12 weeks , fol⁃lowed by sampling. The general conditions and respiratory symptoms of the rats were observed. The pathological changes of lung tissues were observed by hematoxylin⁃eosin (HE) staining technique ; the mRNA and protein ex⁃pression levels of TLR4 , tumor necrosis factor⁃α (TNF⁃α ) , interleukin⁃1β (IL⁃1β) and NLRP3 in rat lung tissueswere detected by real⁃time quantitative polymerase chain reaction (RT⁃qPCR) and Western blot.
Results:Com⁃pared with the normal control group , the lung tissues of rats in the model group were significantly damaged , and the protein and mRNA expression of TLR4 , TNF⁃α , IL⁃1β , and NLRP3 increased ( P < 0. 05 ) . Compared with the model group , lung tissue damage was reduced in the prednisone group and the ginkgo biloba extract group , and TLR4 , TNF⁃α , IL⁃1β , NLRP3 protein and mRNA expression decreased (P < 0. 05) .
Conclusion:Ginkgo biloba airway inflammatory response by inhibiting the TLR4/NLRP3 signaling pathway.
- Full text:2026030721354439761银杏叶提取物下调TLR4_NLRP3信号保护COPD大鼠气道炎症损伤_潘颖.pdf
