Expression of COL1A2 in cervical cancer and its relationship with tumor immune infiltration
10.19405/j.cnki.issn1000-1492.2025.10.005
- VernacularTitle:宫颈癌中 COL1A2 的表达及其与肿瘤免疫浸润的关系
- Author:
Yu Zhang
1
;
Xiaoyu Zhu
1
;
Dianqin Xu
1
;
Xiaowei Chen
2
;
Mingyan Zhong
2
;
Xinzhu Zhou
1
;
Yujie Tan
1
Author Information
1. Center for Clinical Laboratories , The Afiliated Hospital of Guizhou Medical University, Guiyang 550004 ; School of Medical Laboratory Medicine , Guizhou Medical University, Guiyang 550004
2. School of Medical Laboratory Medicine , Guizhou Medical University, Guiyang 550004
- Publication Type:Journal Article
- Keywords:
cervical cancer;
COL1A2;
tumor immunity;
PAX5;
immune infiltration
- From:
Acta Universitatis Medicinalis Anhui
2025;60(10):1808-1817
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the expression of collagen type 1 alpha 2 (COL1A2) in cervical cancer and its correlation with immune infiltration.
Methods:Bioinformatics techniques were used to analyze the expression of COL1A2 in cervical cancer. Western blot and RT-qPCR were used to detect the expression of COL1A2 in cervical cancer tissues and cell lines. The correlation between the expression of COL1A2 and tumor immune cell infiltration was analyzed by tumor immune estimation resource (TIMER2. 0) . Gene set enrichment analysis (GSEA) was used to analyze the possible mechanism of COL1A2 in cervical cancer. Jaspar database was used to predict the transcrip- tion factors of COL1A2. Western blot and RT-qPCR were used to detect the expression of transcription factors in cervical cancer tissues and cell lines.
Results:The expression of COL1A2 was down-regulated in cervical cancer (P < 0. 05) . The expression of COL1A2 was positively correlated with the levels of macrophages and myeloid den- dritic cells (P < 0. 01) . The proportions of 22 types of immune cells were different in different cervical cancer pa- tients. In addition , compared with the high expression group of COL1A2 , the proportion of M0 macrophages , M2 macrophages and resting memory CD4 + T cells increased in the low expression group of COL1A2 , while the propor- tion of CD8 + T cells , activated memory CD4 + T cells , follicular helper T cells , activated NK cells and activated myeloid dendritic cells decreased (P < 0. 05) . GSEA analysis showed that COL1A2 was related to immune-related signaling pathways , including Notch signaling pathway , interleukin-6/janus kinase/signal transducer and activator of transcription 3 (IL6/JAK/STAT3) , Wnt/β-catenin signaling pathway , etc. (P < 0. 01) . Jaspar database pre- dicted that the transcription factor of COL1A2 was paired box protein 5 (PAX5) , and the expression of PAX5 de- creased in cervical cancer (P < 0. 05) .
Conclusion:COL1A2 is expected to become a potential diagnostic biomar- ker and immunotherapy target for cervical cancer.
- Full text:2026030719451689677宫颈癌中COL1A2的表达及其与肿瘤免疫浸润的关系_张瑜.pdf
