Culture and identification of rat corpus cavernosum smooth muscle cells by modified tissue block adherence method
- VernacularTitle:改良组织块贴壁法培养大鼠阴茎海绵体平滑肌细胞及鉴定
- Author:
Tao ZHANG
1
;
Maobin YU
1
;
Meijun LIU
2
;
Ziyang MA
1
;
Peihai ZHANG
1
Author Information
- Publication Type:Journal Article
- Keywords: rats; corpus cavernosum smooth muscle cells; smooth muscle actin; vitro model; cell culture; immunofluorescence; tissue block adherence method
- From: Acta Universitatis Medicinalis Anhui 2026;61(1):113-117
- CountryChina
- Language:Chinese
- Abstract: ObjectiveTo establish an in vitro culture model of rat corpora cavernous smooth muscle cells （CCSMCs） using a modified tissue block adherence method. MethodsCorpus cavernosum smooth muscle tissue was digested with collagenase type I and subsequently cultured using an adherent method. Cells were purified via differential adhesion and identified through immunofluorescence and Western blotting. ResultsCCSMCs began to emerge from the tissue block after 3 days， increased significantly by day 7， and converged by day 12. Post-passage， CCSMCs exhibited strong proliferation and a “peak-to-valley” phenomenon. After purification， the cells tested positive for α-smooth muscle actin （α-SMA）， confirming the successful establishment of the in vitro culture model. ConclusionThe modified tissue block adherence method is a cost-effective and efficient way to obtain high-purity CCSMCs.
