Analysis of the changes in intestinal microbiota of patients with moderate to severe acne based on 16S rRNA high-throughput sequencing technology

Shichao JIANG; Xiaomeng WANG; Zheng CHEN; Song QIAO; Fan YANG; Birong GUO

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Analysis of the changes in intestinal microbiota of patients with moderate to severe acne based on 16S rRNA high-throughput sequencing technology

VernacularTitle:基于16S rRNA高通量测序技术分析中重度痤疮患者的肠道菌群变化
Author: Shichao JIANG ¹ ; Xiaomeng WANG ¹ ; Zheng CHEN ¹ ; Song QIAO ¹ ; Fan YANG ¹ ; Birong GUO ¹
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1. Department of Dermatology， The Third Affiliated Hospital of Anhui Medical University， The First People’s Hospital of Hefei， Hefei　230061
Publication Type:Journal Article
Keywords: acne vulgaris; gut microbiota; 16S rRNA gene; high-throughput sequencing; bacteroidota; biomarker
From: Acta Universitatis Medicinalis Anhui 2026;61(1):98-103
CountryChina
Language:Chinese
Abstract: ObjectiveTo explore the relationship between acne vulgaris and gut microbiota. MethodsA total of 29 clinical cases diagnosed with moderate-to-severe acne vulgaris and 26 healthy individuals as control subjects were recruited. Fecal specimens were collected from all participants， and further analysis of gut microbial communities was performed by leveraging high-throughput sequencing techniques that target the hypervariable regions of 16S rRNA genes. ResultsAssociations between acne vulgaris and alterations in gut microbiota were identified. At the phylum level， the relative abundance of Bacteroidota exhibited a statistically significant elevation in the acne vulgaris cohort when compared with the healthy control group （P<0.01）， while Cyanobacteria was significantly lower in the acne group （P<0.01）. At the genus level， the top five different bacterial taxa in both groups were Bacteroides， Escherichia⁃Shigella， Klebsiella， Roseburia， and Parabacteroides. Among them， Bacteroides， Roseburia， and Parabacteroides were more abundant in acne patients. Linear discriminant analysis identified five biomarkers all belonging to the Bacteroidota phylum in the acne and control groups. These biomarkers belong to the phylum Bacteroidetes. ConclusionThere are significant differences in the composition of intestinal microbiota between acne patients and healthy people. Changes in the richness of specific bacterial genera may become new targets for the diagnosis and treatment of acne.