Microbiological characterization of Staphylococcus epidermidis with hemolytic phenotype
- VernacularTitle:溶血型表皮葡萄球菌的微生物学特征研究
- Author:
Guiyun LENG
1
;
Wei CHEN
1
;
Chenghao WANG
1
;
Jie YAO
1
;
Chuanping CHEN
2
;
Wei TANG
1
Author Information
- Publication Type:Journal Article
- Keywords: Staphylococcus epidermidis; hemolytic phenotype; microbiological characteristics; antibiotic resistance; antagonistic effects; growth curve; toxicity to human erythrocytes
- From: Acta Universitatis Medicinalis Anhui 2026;61(1):60-66
- CountryChina
- Language:Chinese
- Abstract: ObjectiveTo explore the microbiological characteristics of Staphylococcus epidermidis with hemolytic phenotype （SEHP）. MethodsHemolytic phenotype was detected using the three-point inoculation method， involving a total of 5 strains of SEHP and 5 strains of Staphylococcus epidermidis with non-hemolytic phenotype （SENHP） . Bacterial species were identified using the Microflex LT MALDI-TOF mass spectrometer， and a phylogenetic tree was constructed through 16S rRNA sequence alignment. Growth curves were monitored through the microcultivation assay. Biofilm formation ability was assessed by microplate crystal violet staining. Red blood cell toxicity was detected using the microplate method. Antimicrobial susceptibility testing of SEHP and SENHP against commonly used antibiotics was performed using a VITEK 2 GP639 test kit. Antagonistic effects of SEHP and SENHP against Staphylococcus aureus and Corynebacterium striatum were evaluated by the Oxford cup inhibition assay. ResultsCompared with SENHP， SEHP exhibited a marked decrease in growth rate during the late logarithmic phase， accompanied by significant hemolytic toxicity. Additionally， it showed lower resistance rates to levofloxacin and moxifloxacin， and could antagonize Staphylococcus aureus and Corynebacterium striatum. ConclusionThe microbiological characteristics of SEHP differ from those of SENHP in that SEHP demonstrates antagonistic effects against S. aureus and C. striatum.
