Effects of oleic acid-induced lipid droplet synthesis on the proliferation，migration， invasion， and epithelial-mesenchymal transition of osteosarcoma cells
- VernacularTitle:油酸诱导的脂滴合成对骨肉瘤细胞增殖、迁移、侵袭和上皮 - 间质转化的影响
- Author:
Mengting WANG
1
;
Yunlong WANG
1
;
Mengxia LIANG
1
;
Jun LIU
2
;
Erbao BIAN
1
Author Information
- Publication Type:Journal Article
- Keywords: human osteosarcoma cell line; lipid droplet synthesis; lipid metabolism; oleic acid; proliferation; migration; invasion
- From: Acta Universitatis Medicinalis Anhui 2026;61(1):9-15
- CountryChina
- Language:Chinese
- Abstract: ObjectiveTo explore the effects of different concentrations of oleic acid on human osteosarcoma cell lines 143B and HOS， as well as the impacts of the optimal concentration of oleic acid on cellular lipid droplet synthesis and cell functions. MethodsThe 143B and HOS cells were treated with varying concentrations of oleic acid （0， 25， 50， 100， and 200 µmol/L） for 48 hours. Following treatment， oil red O staining and BODIPY staining were performed to determine the optimal concentration. Subsequently， CCK-8 assays and colony formation experiments were conducted to assess the effect of this optimal concentration of oleic acid on the cell proliferation of both cell lines. Transwell migration assays were utilized to evaluate the influence of the optimal concentration on migratory capacity and Transwell invasion assays were utilized to evaluate the invasive ability. Additionally， Western blot analysis was employed to examine the expression levels of epithelial-mesenchymal transition （EMT） markers Epithelial cadherin （E-cadherin） and Neural cadherin （N-cadherin） in response to treatment with the optimal concentration of oleic acid. ResultsTreatment with oleic acid did not induce significant cell death in either 143B or HOS cells； however， an increase in intracellular lipid droplets was observed alongside enhanced proliferation， migration， invasion capabilities as well as EMT transformation potential （P<0.05）. ConclusionOleic acid induces lipid droplet synthesis in osteosarcoma cells which subsequently promotes their proliferation， migration and invasion abilities along with EMT transformation.
