The correlation of TIPE3 expression in colorectal cancer with tumor immune infiltration and the prognosis of patients
10.19405/j.cnki.issn1000-1492.2025.11.024
- VernacularTitle:TIPE3 在结直肠癌中的表达与肿瘤免疫浸润及患者预后的关系
- Author:
Yuan Zhang
1
;
Yue Xu
1
;
Yong Zhu
2
;
Pingping Zhang
1
Author Information
1. Dept of Physiology , School of Basic Medical Sciences , Anhui Medical University , Hefei 230032
2. Dept of General Surgery , The First Afiliated Hospital of Anhui Medical University , Hefei 230022
- Publication Type:Journal Article
- Keywords:
TIPE3;
colorectal cancer;
immune infiltration;
prognosis;
tumor tissue;
tumor normal tissue
- From:
Acta Universitatis Medicinalis Anhui
2025;60(11):2166-2174,2181
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of TIPE3 in colorectal cancer (CRC) and its correlation with tumor immune infiltration and prognosis .
Methods:The expression of TIPE3 in tumor and adjacent normal tissues from 110 CRC patients was detected using real-time fluorescence quantitative enzyme chain reaction ( qPCR) , Western blot , immunohistochemistry ( IHC ) , bioinformatics analysis , and single-cell RNA sequencing (scRNAseq) . The association between TIPE3 expression and clinicopathological factors , prognosis , and immune cell infiltration was analyzed .
Results:Transcriptomic and proteomic analyses revealed that among the TIPE fami- ly , TIPE (P < 0. 05) and TIPE3 (P < 0. 001) were significantly upregulated in CRC tissues compared to adjacent normal tissues , with TIPE3 showing the most pronounced increase . IHC further confirmed that TIPE3 expression was significantly higher in CRC tissues (P < 0. 001) . Kaplan-Meier survival analysis indicated that high TIPE3 ex- pression was associated with reduced survival and poorer prognosis . Immune infiltration analysis demonstrated that patients with high TIPE3 expression exhibited fewer CD8 + T cells and CD20 + B cells but more CD66b + neutro- phils , suggesting a correlation between TIPE3 and unfavorable tumor prognosis . Additionally , scRNA-seq of CRC tissues yielded results consistent with the IHC findings .
Conclusion:TIPE3 expression in CRC is associated with tumor immune infiltration , and its upregulation predicts poor prognosis , indicating its potential as a therapeutic and preventive target in CRC .
- Full text:2026030522524601527TIPE3在结直肠癌中的表达与肿瘤免疫浸润及患者预后的关系_张原.pdf
