Experimental research on LIF promoting lipopolysaccharide-induced pulpal inflammatory response
10.19405/j.cnki.issn1000-1492.2025.11.018
- VernacularTitle:LIF 促进脂多糖诱导的牙髓炎性反应的实验研究
- Author:
Hao Liu
1
;
Youming Zhu
1
;
Song Li
1
Author Information
1. College & Hospital ofStomatology , Anhui Medical University , Hefei 230032 ; Key Lab. of Oral Diseases Research of Anhui Province , Hefei 230032
- Publication Type:Journal Article
- Keywords:
leukemia inhibitory factor;
pulpitis;
immunoinflammation;
LPS;
human dental pulp stem cells;
pulp regeneration
- From:
Acta Universitatis Medicinalis Anhui
2025;60(11):2124-2130
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the role of leukemia inhibitory factor (LIF) in dental pulp inflammation .
Methods:Human dental pulp stem cells (hDPSCs) were cultured in vitro as the target cells , the inflammatory response was induced by lipopolysaccharide (LPS) , and high-throughput sequencing was used to detect relevant highly ex- pressed genes in the inflammatory state . The expression of LIF under graded concentrations of LPS stimulation was detected by real-time fluorescence quantitative PCR (qPCR) . The expression levels of interleukin-6 (IL-6) , inter- leukin-1β(IL-1β) , and tumor necrosis factor-α(TNF-α) were detected after LIF knockdown and overexpression in hDPSCs by qPCR . Normal and inflammatory pulp tissues were collected , and the expression of LIF in both tis- sues was detected by qPCR and immunofluorescence (IF) .
Results:The expression level of LIF increased in hu- man dental pulp cells after LPS stimulation . The expression level of LIF was subsequently elevated in inflammatory pulp induced by graded concentrations of LPS . The expression of IL-6 , IL-1β, and TNF-αwas significantly down- regulated in hDPSCs after LIF knockdown in response to LPS stimulation , while LIF overexpression upregulated the expression of these cytokines . qPCR and IF assays showed high expression of LIF in inflamed pulp tissue .
Conclusion:LIF is involved in dental pulp inflammation and promotes the development of pulpitis .
- Full text:2026030512453118591LIF促进脂多糖诱导的牙髓炎性反应的实验研究_刘昊.pdf
