The correlation between chemerin levels and intestinal mucosal inflammation in IBS-D model mice
10.19405/j.cnki.issn1000-1492.2025.11.014
- VernacularTitle:趋化素水平与 IBS-D 模型小鼠肠黏膜炎症相关性
- Author:
Zhuoyue Xing
1
;
Juan Bai
1
;
Xin Gao
1
;
Jiarui Huang
1
;
Lihong Xu
1
;
Yinfeng Gao
1
Author Information
1. Dept of General Practice , The First Afiliated Hospital of Shihezi University , Shihezi 832000
- Publication Type:Journal Article
- Keywords:
diarrhea-predominant irritable bowel syndrome;
animal model;
chemerin;
intestinal mucosa inflammation;
wire restraint stress
- From:
Acta Universitatis Medicinalis Anhui
2025;60(11):2091-2095
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the correlation of chemerin levels in the serum and intestinal mucosal with in- testinal mucosal inflammation in IBS-D mice .
Methods:A total of 128 female C57BL/6J mice were randomly di- vided into IBS-D group and control group , with 64 mice in each group . Wire Restraint Stress method (WRS) was used to construct an animal model of IBS-D . Enzyme-linked immunosorbent assay was used to detect serum and co- lonic mucosal chemerin levels in mice . Hematoxylin-eosin (HE) staining was used to observe colonic mucosal in- flammation . Geboes value was used to evaluate the severity of colonic mucosal inflammation .
Results:In the IBS- D group , the concentration of serum chemerin increased and reached the peak in the first week of the experiment ( t = 6. 538 , P < 0. 001) , and the concentration of colonic mucosa chemerin increased and reached the peak in the fourth week of the experiment ( t = 8 . 104 , P < 0. 001) ; in the first week of experiment , the colonic mucosa of IBS- D mice showed inflammatory reaction , which was the most significant in the fifth week (P < 0. 05) . Geboes score was ( 1 . 75 ±0. 50) vs (0. 55 ±0. 52) .
Conclusion:There is a temporal sequence between the elevation of serum and intestinal mucosal chemerin levels and the severity of intestinal mucosal inflammation , and it is hypothesized that the elevated serum and intestinal mucosal chemerin levels correlate with the onset and progression of intestinal mucosal inflammation .
- Full text:2026030511153321286趋化素水平与IBS-D模型小鼠肠黏膜炎症相关性_邢卓越.pdf
