Extraction , purification and identification technologies of exosomes derived from Polygonum cuspidatum
10.19405/j.cnki.issn1000-1492.2025.11.012
- VernacularTitle:虎杖来源外泌体的提取 、纯化及鉴定技术
- Author:
Jiayu Wu
1
;
Hong Zhang
2
Author Information
1. Dept of Emergency Medicine , The First Afiliated Hospital of Anhui Medical University , Hefei 230022
2. Dept of Emergency Medicine , The First Afiliated Hospital of Anhui Medical University , Hefei 230022; Dept of Emergency Medicine , The First Afiliated Hospital of Anhui Medical University North District , Hefei 230012
- Publication Type:Journal Article
- Keywords:
Polygonum cuspidatum;
exosomes;
extraction;
purification;
identification
- From:
Acta Universitatis Medicinalis Anhui
2025;60(11):2076-2082
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the extraction,purification,and identification methods of Polygonum cuspidatum(P.cuspidatum)-derived exosomes and to obtain high-purity exosomes,and thus providing a reliable material foundation for their functional studies.
Methods:Exosomes were isolated from fresh P.cuspidatum using ultracentrifugation combined with sucrose density gradient centrifugation.Morphological characteristics,size distribution,and surface charge properties were analyzed by transmission electron microscopy(TEM),nanoparticle tracking analysis(NTA),and Zeta potential measurements.
Results:The purified P.cuspidatum exosomes exhibited characteristic cup-shaped or spherical bilayer morphology with a predominant size distribution of 100-200 nm,consistent with established plant exosome characteristics.The surface of these exosomes exhibits a negative charge,with the Zeta potential absolute value of root-derived exosomes being slightly higher than that of stem/leaf-derived counterparts.
Conclusion:The study successfully isolates and characterizes P.cuspidatum exosomes,with comprehensive analysis confirming their distinctive structural and biophysical properties.It lays a good material foundation for further exploration of its active ingredients and biological functions.
- Full text:2026030511053531996虎杖来源外泌体的提取、纯化及鉴定技术_吴嘉宇.pdf
