Analysis of differential expression of lipid metabolism-related proteins in adipose tissue exosomes from obese mice
10.19405/j.cnki.issn1000-1492.2025.11.011
- VernacularTitle:肥胖小鼠脂肪组织外泌体中 脂质代谢相关蛋白的差异表达分析
- Author:
Xinzhe Zhuo
1
;
Kai Wang
2
;
Yunan Shi
1
;
Xiang Wang
1
;
Jiao Yu
1
;
Jiali Li
1
;
Yidan Liu
3
;
Xiuyun Wang
1
Author Information
1. Dept of Biochemistry , School of Basic Medicine , Anhui Medical University , Hefei 230032
2. Dept of Biochemistry and Clinical Laboratory , School of Medical Technology , Anhui Second Medical College , Hefei 230601
3. Dept of Biochemistry and Molecular Biology , School of Life Sciences , Anhui Medical University , Hefei 230032
- Publication Type:Journal Article
- Keywords:
obesity;
adipose tissue;
exosome;
proteomic analysis;
differentially expressed proteins;
lipid metabolism-related proteins
- From:
Acta Universitatis Medicinalis Anhui
2025;60(11):2069-2075
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze differences in the expression levels of lipid metabolism-related proteins in adi- pose tissue exosomes between obese mice and wild-type mice using proteomic techniques .
Methods:Wild-type (WT) and obese (ob/ob) model mice of the same age were selected , with 8 mice per group . Adipose tissue from both groups was minced and cultured for 48 hours . Conditioned media was collected , and exosomes were isolated u- sing differential centrifugation . Liquid chromatography-tandem mass spectrometry ( LC-MS/MS) was utilized for proteomic analysis to screen differentially expressed proteins ( DEPs) . Gene ontology ( GO) enrichment analysis and kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analysis were performed on the DEPs . Heatmaps were generated to visualize DEPs expression patterns , and Western blot was employed to validate DEPs expression levels .
Results:Exosomes were successfully extracted from the culture supernatant of adipose tissues from mice in the WT group and the ob/ob group . Mass spectrometry analysis identified a total of 25 629 peptides and 3 376 proteins . Compared with the WT group , there were 699 proteins with high expression and 632 proteins with low expression in the exosomes derived from adipose tissues of ob/ob mice . Both GO and KEGG analyses showed that DEPs were mainly enriched in metabolic pathways . Heatmap analysis visualized the expression patterns of metabolism-related DEPs , and the expression levels of lipid metabolism-related proteins such as acyl-CoA syn- thetase long-chain family member 1 (ACSL1) , apolipoprotein E (ApoE) , and albumin (Alb) changed significant- ly in the obese state (P < 0. 05) . Western blot verification results showed that the expression of ApoE and Alb pro- teins in the adipose tissue-derived exosomes of ob/ob mice decreased ( P < 0. 01) .
Conclusion:In the obese state , the expression levels of lipid metabolism-related proteins in mouse adipose tissue exosomes are significantly altered . These differentially expressed proteins may thus serve as potential molecular targets for treating obesity and its associated metabolic complications .
- Full text:2026030511005244120肥胖小鼠脂肪组织外泌体中脂质代谢相关蛋白的差异表达分析_禚鑫喆.pdf
