Effect of acarbose and pioglitazone on hypothalamic-pituitary-ovarian axis
10.19405/j.cnki.issn1000-1492.2025.07.012
- Author:
Rong Xiao
1
;
Yan Xue
1
;
Xiaoman Gao
1
;
Yaqi Zhang
1
;
Lin Wang
2
;
Datong Deng
1
Author Information
1. Dept of Endocrinology，The First Affiliated Hospital of Anhui Medical University，Hefei 230022
2. Dept of Infection，The First Affiliated Hospital of Anhui Medical University，Hefei 230022
- Publication Type:Journal Article
- Keywords:
hypothalamic-pituitary-ovarian axis;
acarbose;
pioglitazone;
rat;
sex hormone;
ovarian hormone
- From:
Acta Universitatis Medicinalis Anhui
2025;60(7):1246-1251
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the effects of acarbose and pioglitazone on the levels of sex hormones in adult female rats ，and to explore their regulatory effects on the hypothalamic-pituitary-ovarian axis ( HPOA) and its mechanism．
Methods :Adult female SD rats aged 2 months were randomly divided into 0．9% sodium chloride so- lution control group (NS) ，acarbose low (AL) ，medium (AM) and high (AH) dose groups and pioglitazone low (PL) ，medium (PM) and high (PH) dose groups．AL，AM and AH groups were given 20，40 and 60 mg / (kg · d) acarbose，and PL，PM and PH groups were given 5，10 and 15 mg / (kg · d) pioglitazone，respectively．Af- ter 28 days of intervention，serum gonadotropin (FSH，LH) and ovarian secreted hormone ( E2，P，T) were de- tected by ELISA，and the effects of acarbose and pioglitazone on HPOA were investigated by organ index analysis and histopathological observation of pituitary gland and ovary．
Results : Compared with the NS group，the serum FSH in the PL，PM and PH groups decreased ( all P＜0. 01) ，the serum LH in the PL，PM and PH groups de- creased (P＜0. 05，P＜0. 01，P ＜0. 01) ，and the organ indices in the PM and PH groups decreased ( all P < 0. 01) ．HE staining of the adenohypophysis in the PL，PM and PH groups showed an increase in eosinophils，a de- crease in basophils，and an expansion and increase in the number of blood sinuses．In the PM and PH groups，HE staining of the ovaries showed that there were no significant differences in follicles at all levels，but the development was poor，the number of primordial follicles decreased，and the number of corpus luteum increased．Compared with the NS group，the serum P in the AM and AH groups decreased ( all P ＜0. 01) ，and serum E2 in AM and AH groups decreasd (all P＜0. 05) ．
Conclusion :Pioglitazone affects HPOA by regulating pituitary function directly． However，the effect of acarbose on HPOA may be mediated through the gut-brain axis，and has no direct correlation with the ovarian structure．
- Full text:2026030509293529935阿卡波糖与吡格列酮对下丘脑-垂体-卵巢轴的影响_肖融.pdf
