Construction of a lentiviral cell line with overexpression of human HACE1 and its impact on the proliferation and migration of glioma cells
10.19405/j.cnki.issn1000-1492.2025.07.011
- Author:
Ran Huang
1
;
Ming Shan
2
;
Weisong Li
1
Author Information
1. Dept of Pediatric Surgery，The First Affiliated Hospital of Anhui Medical University，Hefei 230022
2. Dept of Neurosurgery，The First Affiliated Hospital of Anhui Medical University，Hefei 230022
- Publication Type:Journal Article
- Keywords:
HACE1;
over-expression;
lentiviral vector;
glioma;
proliferation;
migration
- From:
Acta Universitatis Medicinalis Anhui
2025;60(7):1241-1245
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To construct a lentiviral overexpression cell line of HACE1 and to investigate its effects on the proliferation and migration of glioma cells．
Methods : Using the bacterial liquid containing the full-length cDNA sequence of human HACE1 as a template，the lentiviral expression plasmid pCDH-HACE1 was constructed，and the lentiviral cell line was screened．Western blot was employed to detect the expression of HACE1 in glioma cells． Meanwhile，the CCK-8 assay and the cell scratch assay were utilized to assess the impacts of HACE1 on the prolif- eration and migration of glioma cells．
Results :The lentiviral cell line with HACE1 overexpression was successfully established．Western blot analysis demonstrated that HACE1 could be effectively expressed in eukaryotic cells．The results of the CCK-8 assay showed that，compared with the control group，the cell proliferation in the HACE1 over- expression group was significantly enhanced at 24 hours and 48 hours after cultivation (both P＜0. 01) ．The cell scratch assay revealed that，at 24 hours and 48 hours after cultivation，the Scratch areas in the HACE1 overexpres- sion group were higher than those in the control group (P＜0. 05，P＜0. 01) ．
Conclusion :The lentiviral cell line with HACE1 overexpression is successfully constructed．HACE1 can promote the proliferation and migration abili- ties of U251 glioma cells，providing a foundation for further research．
- Full text:2026030509261819053人HACE1过表达慢病毒细胞株构建及其对胶质瘤细胞增殖迁移的影响_黄冉.pdf
