Expression and significance of kynurenine-3-monooxygenase in peripheral blood mononuclear cells and synovial tissue of patients with rheumatoid arthritis
10.19405/j.cnki.issn1000-1492.2025.07.008
- Author:
Xuemei Zong
1
;
Qianqian Lin
1
;
Yuelan Chen
1
;
Xinming Wang
2
;
Wei Wei
1
;
Shangxue Yan
3
;
Yan Chang
3
Author Information
1. Institute of Clinical Pharmacology，School of Pharmaceutical Sciences，Anhui Medical University， Key Laboratory of Anti-inflammatory and Immunological Drugs，Ministry of Education，Anhui Collaborative Innovation Center for Anti-inflammatory and Immune Drugs，Hefei 230032
2. Dept of Pharmacy， The First Affiliated Hospital of Anhui Medical University，Hefei 230022
3. Institute of Clinical Pharmacology，School of Pharmaceutical Sciences，Anhui Medical University， Key Laboratory of Anti-inflammatory and Immunological Drugs，Ministry of Education，Anhui Collaborative Innovation Center for Anti-inflammatory and Immune Drugs，Hefei 230032; Laboratory Animal Center，Anhui Medical University，Hefei 230032
- Publication Type:Journal Article
- Keywords:
rheumatoid arthritis;
kynurenine 3-monooxygenase;
kynurenine;
3-hydroxykynurenine;
peripheral blood mononuclear cells;
synovial tissue;
fibroblast-like synoviocytes
- From:
Acta Universitatis Medicinalis Anhui
2025;60(7):1218-1224
- CountryChina
- Language:Chinese
-
Abstract:
Objective : To investigate the expression and clinical significance of kynurenine-3-monooxygenase (KMO) in peripheral blood mononuclear cells ( PBMC ) ，synovial tissue ，and fibroblastic-like synovial cells (FLS) of rheumatoid arthritis (RA) patients．
Methods :Peripheral blood samples from 25 healthy control ( HC) individuals and 25 patients diagnosed with RA were collected ，and real-time fluorescence quantitative PCR and Western blot were used to detect KMO gene and protein expression in PBMC of RA and HC groups，and to analyze the correlation between the expression level of the KMO gene in the PBMC of the RA patients and the indexes of the laboratory tests．Meanwhile，immunohistochemistry and immunofluorescence were used to detect KMO expression in synovial tissue and FLS in RA and HC groups．
Results :① KMO gene and protein expression in PBMC of RA group were higher than that of HC group，and the difference was statistically significant (P＜0. 001) ．② The level of KMO gene expression in PBMC of RA group was positively correlated with disease activity index 28 score，blood sedimentation，and rheumatoid factor (rs = 0. 417，P = 0. 038 ; r = 0. 545，P = 0. 005 ; rs = 0. 433，P = 0. 031) ， and had no correlation with C-reactive protein and anti-cyclic citrullinated polypeptide antibody．③ KMO expres- sion in synovial tissue of RA group was higher than that of HC group，and the difference was statistically significant (P＜0. 01) ; KMO expression in FLS of synovial tissue of RA group was higher than that of HC group，and the difference was statistically significant (P ＜0. 001) ．
Conclusion:KMO expression increases in PBMC ，synovial tissue and FLS of RA patients，and the level of KMO gene expression is correlated with the disease activity of RA patients，suggesting that KMO may promote the course of RA．
- Full text:2026030509155585071犬尿氨酸-3-单加氧酶在类风湿关节炎患者外周血单个核细胞和滑膜组织中的表达及意义_宗雪梅.pdf
