The influence of enriched environment on cognitive impairment and GAP-43 content changes in pregnant mice with obstructive sleep apnea-hypopnea syndrome through regulating the NF-κB/NLRP3 pathway
10.19405/j.cnki.issn1000-1492.2025.07.007
- Author:
Yunzhou Cheng
1
;
Xueyan Li
2
;
Yueming Zhang
2
;
Rumeng Wei
2
;
Yang Wang
3
Author Information
1. Dept of Pediatrics，The Affiliated Chaohu Hospital of Anhui Medical University，Hefei 238000
2. Dept of Sleep Disorders，The Affiliated Chaohu Hospital of Anhui Medical University，Hefei 238000
3. Dept of Neonatology，The First Affiliated Hospital of Anhui Medical University，Hefei 230022
- Publication Type:Journal Article
- Keywords:
enriched environment;
OSAHS;
cognitive impairment;
GAP-43;
NF-κB;
NLRP3
- From:
Acta Universitatis Medicinalis Anhui
2025;60(7):1212-1217
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the effect of environmental enrichment on cognitive impairment and hippo- campus GAP-43 changes induced by exposure to obstructive sleep apnea-hypopnea syndrome ( OSAHS) during the period of late pregnancy in mice，and to explore relative inflammatory pathway mechanism．
Methods :The experi- mental group of C57BL/6J pregnant mice were exposed to an intermittent hypoxic environment for 7 consecutive days starting from gestational day 15．The corresponding offspring were then placed in an enriched environment from postnatal day 21 to 2 months of age (designated as OSAHS + EE group) or in a normal environment (designat- ed as OSAHS group) ．Pregnant mice in the control group were maintained in a normal oxygen environment，and their corresponding offspring were placed in an enriched environment (designated as Control + EE group) or a nor- mal environment (designated as Control group) at the same ages．The spatial learning and memory ability of the mice was assessed by Morris water maze at the age of 2 months．The mRNA levels of NF-kB，NLRP3 and GAP-43 in the hippocampus were detected by real-time fluorescence quantitative PCR , and the protein levels of NLRP3 and GAP-43 in the hippocampus were detected by Western blot．
Results:Compared with Control group，the swimming distance increased (P＜0. 01) ，and the percentage of swimming distance in target quadrant decreased (P＜0. 01) in OSAHS group．The level of NF - κB mRNA，NLRP3 mRNA and protein in the hippocampus was increased，and the level of GAP-43 mRNA and protein was decreased (P＜0. 01) ．Compared with the Control group，there were no significant differences in swimming distance，percentage of swimming distance，NF-κB mRNA，NLRP3 mRNA and protein content in the OSAHS + EE group．
Conclusion :OSAHS during pregnancy impairs the learning and memory ability of offspring mice and reduces the level of GAP-43 protein．The mechanism may be related to the in- crease of NF-κB / NLRP3 level，and environmental enrichment can improve the damage．
- Full text:2026030417291375623丰富环境通过调节NF-κB_NLRP3通路对孕期OSAHS小鼠认知功能损害和GAP-43含量变化的影响_程云舟.pdf
