Solasonine regulate the effect of STAT3 signaling pathway on biological behavior of lung adenocarcinoma cells
10.19405/j.cnki.issn1000-1492.2025.07.004
- Author:
Beibei Ma
1
;
Lianyu Cheng
1
;
Zhongwei Zhang
2
;
Guangbin Ye
1
;
Yanli Li
1
;
Bo Ling
1
Author Information
1. School of Basic Medical Sciences，Youjiang Medical University for Nationalities，Baise 533000
2. School of Pharmacy， Youjiang Medical University for Nationalities，Baise 533000
- Publication Type:Journal Article
- Keywords:
solasonine;
lung adenocarcinoma;
H1299;
apoptosis;
migration;
STAT3
- From:
Acta Universitatis Medicinalis Anhui
2025;60(7):1187-1193
- CountryChina
- Language:Chinese
-
Abstract:
Objective : To investigate the effect of solasonine regulation of the STAT3 signaling pathway on the bio- logical behavior of lung adenocarcinoma cells．
Methods :H1299 cells were treated with 0. 125，0. 25，0. 5 and 0. 75 mmol /L solasonine，respectively．The proliferative activity of H1299 cells was detected by CCK-8．The mi- gration and invasion ability of H1299 cells were detected by scratch，Transwell migration and invasion assay．The apoptosis level of H1299 cells was detected by flow cytometry and Hoechest 33258 /PI double staining．The protein expression levels of STAT3，p-STAT3 ，Bcl-2 ，Bax ，Caspase-3 ，Cl-Caspase-3 ，Snail ，Slug ，N-cadherin and E- cadherin in H1299 cells were detected by Western blot assay．
Results:Solasonine at different concentrations sig- nificantly reduced the proliferation of H1299 cells (P＜0. 05) ．0. 125 and 0. 25 mmol /L solasonine promoted the apoptosis of H1299 cells (P＜0. 05) and inhibited the migration and invasion of H1299 cells (P＜0. 05) ．Solaso- nine inhibited the expression of STAT3，p-STAT3 and Bcl-2 proteins，enhanced the expression of Bax，Caspase-3 and Cl-Caspase-3 proteins．Solasonine inhibited the activation of STAT3 in cells，reduced Snail and Slug protein expression levels，enhanced E-cadherin，reduced N-cadherin(P＜0. 05) ．
Conclusion :Solasonine can inhibit the activation of STAT3 ，activate the Bcl-2 /Bax / Caspase3 apoptosis pathway ，inhibit the continuous proliferation of lung adenocarcinoma H1299 cells，and promote the apoptosis of lung adenocarcinoma H1299 cells．Meanwhile，it can inhibit the activation of STAT3，reduce the expression of Snail / Slug protein，affect the EMT transformation of lung adenocarcinoma H1299 cells，and inhibit the migration and invasion of lung adenocarcinoma H1299 cells．
- Full text:2026030417194368367澳洲茄碱调控STAT3信号通路对肺腺癌细胞生物学行为的影响_马贝贝.pdf
