Correlation between TRIM21 and Cisplatin induced acute kidney injury in mice
10.19405/j.cnki.issn1000-1492.2025.11.003
- VernacularTitle:TRIM21 与顺铂诱导小鼠急性肾损伤的相关性
- Author:
Qingqing Sun
1
;
Lei Ni
1
;
Wei Wei
1
;
Chun Wang
1
Author Information
1. School of Pharmacy and Science , Anhui Medical University , Hefei 230032
- Publication Type:Journal Article
- Keywords:
acute kidney injury;
cisplatin;
TRIM21;
different time points;
endoplasmic reticulum stress;
renal pathological score
- From:
Acta Universitatis Medicinalis Anhui
2025;60(11):2005-2010
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of triple motif protein 21 (TRIM21) at different time points in Cisplatin induced acute kidney injury (Cis-AKI) mouse model and its correlation with pathological score .
Methods:Acute kidney injury (AKI) model was induced by intraperitoneal injection of cisplatin (20 mg/kg) . Peripheral blood serum was collected at 12 , 24 , 48 and 72 h , respectively . Body weight and kidney mass were also recorded . The dynamic changes of serum creatinine ( SCr) and urea nitrogen ( BUN) were detected . The expressions of TRIM21 protein and endoplasmic reticulum stress (ERS) proteins GRP78 , GRP94 , P-elf2αand CHOP were de- tected by Western blot. HE staining was used to observe the pathology of renal tissue and analyze the correlation between the expression of TRIM21 protein at different time points .
Results:Compared with the control group , the renal body ratio of Cis-AKI mice significantly increased at 48 h and 72 h (P < 0. 01) . SCr and BUN of Cis-AKI mice significantly increased at 48 h and 72 h compared with control group ( P < 0. 01) . HE staining showed that compared with the control group , the renal pathological scores of Cis-AKI mice increased at 12 and 24 h ( P < 0. 000 1) . At the same time , the expression of TRIM21 protein significantly increased at 24 h (P < 0. 01) . Com- pared with the control group , the renal pathological score of Cis-AKI mice significantly increased at 48 h ( P < 0. 000 1) , and the protein expression of TRIM21 , CHOP and GRP78 was significantly up-regulated at 48 h (P < 0. 05) . Compared with the control group , the renal pathological score of Cis-AKI mice significantly increased at 72 h (P < 0. 000 1) , and the expression of TRIM21 , GRP94 and P-elf2αproteins was significantly up-regulated at 72 h (P < 0. 05) . The results of correlation analysis showed that the expression of TRIM21 protein at different time points was positively correlated with the HE pathological scores at different time points (P < 0. 000 1) .
Conclusion:At different time points , TRIM21 may participate in the pathological progression of Cis-AKI by affecting ERS ,and it is positively correlated with renal pathological scores , which is expected to become a potential early diagnos- tic marker and intervention target.
- Full text:2026030411363716653TRIM21与顺铂诱导小鼠急性肾损伤的相关性_孙晴晴.pdf
