Antibacterial properties and compatibility of plasma-activated water/bletilla striata polysaccharide composite hydrogel
10.19405/j.cnki.issn1000-1492.2025.11.002
- VernacularTitle:等离子体活化水协同白及多糖制备水凝胶的 抗菌性能及相容性
- Author:
Wenxue Ren
1
;
Cheng Cheng
2
;
Wei Han
3
;
Taofeng Liu
4
Author Information
1. The First Clinical Medical College of Anhui University of Chinese Medicine , Hefei 230001
2. Institute of Plasma Physics , Hefei Institutes of Physical Science , Chinese Academy of Sciences , Hefei 230031
3. Institute of Health and Medical Technology , Hefei Institutes of Physical Science , Chinese Academy of Sciences , Hefei 230031
4. Dept of Dermatology , The First Afiliated Hospital of Anhui University of Chinese Medicine , Hefei 230001
- Publication Type:Journal Article
- Keywords:
plasma-activated water;
bletilla striata polysaccharide;
hydrogel;
wound healing;
antimicrobial;
cytotoxicity
- From:
Acta Universitatis Medicinalis Anhui
2025;60(11):1996-2004
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the antibacterial properties and biocompatibility of a novel hydrogel fabricated by integrating plasma-activated water(PAW) and bletilla striata polysaccharide(BSP).
Methods:The experiments were carried out by dividing the hydrogels into four groups based on their compositions:the deionized water(DW)-carbomer 940(CBM940)-carboxymethyl chitosan(CMCS) hydrogel group(H group),the PAW-CBM940-CMCS hydrogel(PAH) group,the DW-BSP-CBM940-CMCS hydrogel(BSPH) group,and the PAW-BSP-CBM940-CMCS hydrogel(PA/BSPH) group.Physical properties of the hydrogels were evaluated by testing water loss rate and water vapor transmission rate.The content of active substances was determined using a microplate reader and an electron spin resonance spectrometer(ESR).The pH and ORP values were measured using a pH meter and an oxidation-reduction potential(ORP) electrode.The antibacterial mechanism was elucidated by analyzing the integrity of bacterial biofilms.Antibacterial activity was evaluated via the zone of inhibition assay.Cytotoxicity testing was performed using the cell counting kit-8(CCK-8) assay,and combined with the cell scratch assay,to collectively evaluate the effects of the hydrogel on cellular biocompatibility and migration ability.
Results:The water loss rate and water vapor transmission rate of PA/BSPH hydrogel were (32. 3 ±2. 3)% and (2 228. 2 ±1 81 . 1) g/( m2 ·d) . Compared with the H group hydrogel , the contents of hydrogen peroxide ( H2 O2 ) and hydroxyl radicals ( ·OH) in PAH and PA/BSPH groups significantly increased (P < 0. 05) , and the diameters of inhibition zones significantly enlarged (P < 0. 05) . The cell viability in PAH group significantly decreased (P < 0. 05) . The PA/ BSPH group showed non-toxicity and a higher cell migration rate ( P < 0. 05 ) .
Conclusion:The antibacterial mechanism of the PA/BSPH hydrogel relies on the reactive species H2 O2 and ·OH in PAW. The incorporation of BSP enhances the water retention and breathability of the wound dressing while significantly reducing the cytotoxici- ty of PAW. This modification endows the hydrogel with improved biocompatibility and promotes cell proliferation . The PA/BSPH hydrogel demonstrates clinical potential by offering a novel therapeutic strategy for chronic infected wound management.
- Full text:2026030411302202718等离子体活化水协同白及多糖制备水凝胶的抗菌性能及相容性_任文雪.pdf
